FastQCFastQC Report
Mon 26 Jun 2023
ERR10323210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15060474
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA1403810.9321154168188863No Hit
CGTTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA804220.5339938171932703No Hit
AAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA765810.5084899718295719No Hit
CGTATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA639140.4243823932765994No Hit
CGCTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA483390.32096599350060295No Hit
TAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA414700.27535653924305437No Hit
AGGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA392840.260841723839502No Hit
CGTGTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA375430.2492816627152638No Hit
GAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA374880.2489164683661351No Hit
CGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA370270.2458554757307107No Hit
CCCCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG363440.24132042590425773No Hit
CGCCTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA359940.23899646186434767No Hit
CGTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAAC355200.23584915056458383No Hit
AGCACATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG283100.18797549134243718No Hit
CGCCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG280820.18646159476786722No Hit
CGATTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA263180.17474881600672065No Hit
GTAGATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGGA251010.16666806104509063No Hit
AGCGCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG245810.16321531447150997No Hit
CGCATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA245060.16271732217724358No Hit
AGCTCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG237640.1577905184126343No Hit
GCCCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG235960.15667501567347747No Hit
CGTCTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA234370.15561927200963263No Hit
CCCGCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG223000.14806970882855347No Hit
CGTAAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAAC210610.13984287612727195No Hit
CCCGTAGGTCAGCATCAGTTTTCGGGGGCGGAAAAAGAATAAGGGAAGGT207000.13744587321753618No Hit
CGGTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA205250.13628389119758116No Hit
CCCACGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG204240.1356132615746357No Hit
AGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA202700.1345907173970753No Hit
CCCCTAGGTCAGCATCAGTTTTCGGGGGCGGAAAAAGAATAAGGGAAGGT194830.12936511825590613No Hit
CGCTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAAC194620.12922568041351157No Hit
CGACTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA190160.1262642862369405No Hit
GCACATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGGA180170.11963102887731156No Hit
CGCGTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA177910.11813041209725537No Hit
CCCTCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG172690.11466438572916098No Hit
ACCGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA164580.1092794290538266No Hit
TGTAATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGGA160420.10651723179496209No Hit
CGAATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA158190.10503653470667657No Hit
CAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA155400.10318400337200544No Hit
AGCCCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG153470.10190250320142648No Hit
CGTTTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGA153280.10177634515354562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)