FastQCFastQC Report
Mon 26 Jun 2023
ERR10323212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR10323212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10781746
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA1171011.086104235807447No Hit
CGTTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA624110.5788580068571454No Hit
AAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA565600.5245903585560261No Hit
CGTATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA479260.444510564429917No Hit
CGCTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA359080.33304438817237947No Hit
TAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA325790.30216812749994293No Hit
GAGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA308320.28596481497523685No Hit
CCCCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG299870.2781274943779978No Hit
AGGGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA295990.27452881935820045No Hit
CGTGTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA287010.2661999271732055No Hit
CGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA272440.2526863459777294No Hit
CGCCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG269010.2495050430607436No Hit
CGTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAAC258430.2396921611768632No Hit
CGCCTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA249640.23153949276861094No Hit
AGCACATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG232230.21539182985761302No Hit
CCCGCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG226360.20994744264982687No Hit
GCCCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG207990.19290938591949763No Hit
AGCGCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG201970.18732587467744094No Hit
CCCGTAGGTCAGCATCAGTTTTCGGGGGCGGAAAAAGAATAAGGGAAGGT195050.18090761922976112No Hit
CCCACGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG192030.17810658867311474No Hit
AGCTCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG184210.17085358901981182No Hit
CGATTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA182370.1691470008660935No Hit
CCCTCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG177860.16496400490236No Hit
CGCATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA175180.16247832215672675No Hit
CGTCTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA174650.1619867505689709No Hit
GTAGATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGGA171530.1590929706561442No Hit
CGGTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA165760.15374133280453833No Hit
CCCCTAGGTCAGCATCAGTTTTCGGGGGCGGAAAAAGAATAAGGGAAGGT162860.15105160147530836No Hit
CGTAAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAAC151930.14091409684479675No Hit
CCCCGCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTC148150.13740817118117976No Hit
AGGTTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA136250.1263709977957188No Hit
CCCGGCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTC133920.12420993779671677No Hit
CGCGTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA133790.12408936363368234No Hit
CGCTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAAC131220.1217057051798475No Hit
CCTACGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG131120.12161295582366716No Hit
CGACTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA130460.12100081007287687No Hit
CCTCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG123340.11439705591283639No Hit
CGTTTTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGA123110.11418373239362159No Hit
CGGCTAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA122940.11402605848811499No Hit
CTCCCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG122710.11381273496890022No Hit
AGCCCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG117990.10943496535718797No Hit
CCACCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTCG115840.10744085419931058No Hit
CGAATAAAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAA113940.10567861643188403No Hit
CAGCCATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGG112940.1047511228700806No Hit
GCACATGCTGAATCCGGTGCGCTCTCGACGGCCCTTGAAAATCCAACGGA110720.10269208716287695No Hit
CCCTGCGAGACTATGCACTCTGAACCTGTGGTAGGTCAGCATCAGTTTTC110600.10258078793546055No Hit
ACCGTACGCCGGTGAAATACCACTACCTTTATCGTTTCTTTACTTAATCA110000.10202429179837849No Hit
CCCATGCCTGGGGAGTGTTTATAGCCCTTGTTGTAATACGTCCACTGGGG107930.10010438012544537No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)