FastQCFastQC Report
Fri 23 Jun 2023
ERR1191012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1191012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68573681
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length1-49
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC12849151.8737728254663768No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG8251941.2033683885221211No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT7795601.1368209911321516No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC7209241.051312966559284No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT6139030.8952458013738537No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC5806240.8467155204924758No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA4977200.7258178250632339No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA4697690.685057288961927No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC4618370.6734901689177222No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG4558630.6647783717487764No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4418340.6443200854275273No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4406530.6425978503326955No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC4349150.6342302085256295No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT4071220.5937000815225305No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG3665680.5345607741255716No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAT3528150.5145049745834702No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT3138880.45773829758387913No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCC3006820.438480180172915No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCG2921700.42606725457832717No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTT2791660.40710371082456553No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCT2784590.40607270302435705No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGT2763640.40301759504495605No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT2736120.39900439353693146No Hit
GCCGCCTGGATACCGCAGCTAGGAATAA2510690.36613026505023116No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCT2456780.3582686482879634No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2379320.34697276933405397No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG2304570.3360720857321339No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC2269180.3309112135893653No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC2214790.3229795991263762No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGAT2153900.31410009913278536No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCAT2122110.309464209745427No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGA2071220.3020429951835311No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACT2058130.3001340995534424No Hit
GAAGCTACCATCTGTGGGATTATGACTGAAC2031770.29629005915549433No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC1987140.2897817312738396No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACA1984110.28933987078803597No Hit
AATAATCCACCTATAACTTCTCTGTTAACCCAA1944830.28361172561233805No Hit
TAGCTCTTTCTCGATTCCGTGGGTGGTGGT1935020.2821811476038453No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT1873550.27321706705521615No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1857890.27093339206918177No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAA1830710.26696977226583474No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG1795190.2617899424124541No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATG1772240.2584431773467141No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTC1735930.2531481429442296No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1701220.24808643421081622No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAG1660690.24217600335615644No Hit
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC1655730.2414526937820357No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG1652120.2409262527411938No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT1634620.23837425323572758No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1600900.23345691476005204No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT1595780.2327102726190242No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTA1595220.2326286086348493No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCT1587050.23143718943715447No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTG1536270.22403201601500725No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1513600.22072608294135473No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC1424580.20774442602840584No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGAC1381360.2014417163926201No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1342970.195843358620343No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCC1337090.19498588678650633No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGA1335860.19480651767840784No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGC1260490.18381542037972265No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA1253950.18286170170739413No Hit
AATAATCCACCTATAACTTCTCTGTTAACCCA1247630.1819400653145629No Hit
AATAATCCACCTATAACTTCTCTGTTAACCCAACA1189020.1733930543993985No Hit
CATAAACGATGCCGACTGGCGATGCGGCGGCG1179120.17194935182202048No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGA1152210.16802510572532922No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCT1150480.167772822345646No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGT1117320.16293714785414537No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT1116350.16279569416727097No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAATG1089410.15886707321428464No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGA1081270.1576800288728849No Hit
AATAATCCACCTATAACTTCTCTGTTAACCCAAC1074260.15665777078526674No Hit
TAGTGAGGCCCTCGGATCGGCCCCGCCGGGG1073310.15651923366925571No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA1057100.1541553529844781No Hit
AGCTAGGAATAATGGAAT1053780.15367120222115538No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1033000.15064088509409318No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGAAC1013470.14779285364599284No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATC1007330.1468974663909321No Hit
CCTGGCGGAGCGCTGAGAAGACGGTCGAACT1003790.1463812333481121No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGC1000430.14589124944306256No Hit
TGACCCGCCGGGCAGCTTCCGGGAAACCAAAGT998410.14559667578586016No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA993490.14487919935346624No Hit
TAGTGAGGCCCTCGGATCGGCCCCGCCGGG991750.1446254576883513No Hit
GGAATGCCTAAAGGAAAGATCCAAAAAGAT983960.14348945333706092No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC963200.1404620527808621No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG955180.1392925078646427No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGA926270.13507660468161248No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGG924920.13487973614833365No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCC922760.13456474649508754No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGG919490.13408788715892325No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATG910870.13283084511680218No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG907690.13236711034952317No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAAC900500.13131860312413446No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT895120.13053404556188256No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCT894760.13048154728634154No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGG893740.13033280217230866No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAG887810.1294680389113135No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACA879900.12831453513484276No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAG868480.12664917317184707No Hit
TAAGTCAGAATCCCGCCCAGGCGGAACGATAC868240.1266141743214864No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGAT847200.12354594177320014No Hit
TGTCAAAGTGAAGAAATTCAATGAAGCGCGGGT845000.12322511897822723No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC843280.12297429388397568No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACA819980.11957648882812634No Hit
CATCGTTTTTTCACTGACCCGGTGAGGCGGGG808440.11789362743995031No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACA800010.11666429282103144No Hit
GGAATGCCTAAAGGAAAGATCCAAAAAGATA798250.11640763458505311No Hit
TGAAAGTCAGCCCTCGACACAAGGGTTTGT782650.11413270931160892No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACA779640.11369376539666871No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC773760.11283629356283206No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT772170.11260442617919257No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAACA769320.11218881483115949No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAT767990.11199486286874406No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAA765550.11163904122341048No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTT765320.11160550065848149No Hit
AACGATGCCGACTGGCGATGCGGCGGCG764790.11152821153060166No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAC757440.11045637173830583No Hit
AAGCTACCATCTGTGGGATTATGACTGAAC740620.10800353564219486No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG732930.1068821141452214No Hit
CCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA727790.10613255543333018No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTG712420.10389117072481496No Hit
CGAAGCTACCATCTGTGGGATTATGACTGAAC699940.10207123050605961No Hit
AGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGA694700.10130708893985142No Hit

[OK]Adapter Content

Adapter graph