FastQCFastQC Report
Thu 22 Jun 2023
ERR1191025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1191025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27122370
Total Bases839.9 Mbp
Sequences flagged as poor quality0
Sequence length1-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGAGTCCACTTTAAATCCTTTAACGA1699630.6266524643679737No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTC1654220.609909827201679No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1547070.5704036925976602No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG990650.3652520041574538No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCG711940.2624918102658433No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACA673650.2483743124218127No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAATG648170.23897985316179965No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC620880.2289180480909301No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT530580.19562449741670807No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC524370.19333487449658715No Hit
GAGGTGGAGCACGAGCGTACGCGTTAGGACCCGA498840.18392198026942336No Hit
AACGATTAAAGTCCTACGTGATCTGAGTT495210.1825836016542802No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG491660.18127471898657824No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGT464740.17134933267262412No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCT445540.16427030528674302No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC438440.16165253995133907No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATT434890.1603436572836371No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT421690.15547682595584383No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCT400480.1476567128905033No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCC390680.14404345932895982No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT388310.14316964188601514No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTT385130.14199717797522857No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG378600.13958957126534297No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA372790.1374474280824279No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC368440.1358435859403142No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCT367880.135637114308226No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAA367290.1354195816958474No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG365970.13493289856306806No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAT360560.13293823511735886No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGAC359900.13269489355096917No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAG358050.1320127997663921No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC354680.13077028298043278No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT353030.13016192906445861No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC347570.12814883065159866No Hit
GGAATGCCTAAAGGAAAGATCCAAAAAGAT346970.12792761104578987No Hit
GTCCGGTGCGGAGAGCCGTTCGTCTTGGGA341570.12593663459351082No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT339150.12504438218341538No Hit
TGGCGCTAAACCATTCGTAGACGACCTGCTT328760.12121359600949326No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC327560.12077115679787571No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCA323970.11944752615645315No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATT317510.11706572840057855No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACA308420.11371425137257547No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAC298120.10991664813952468No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC293750.10830543201055069No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA290770.10720670796836708No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAA290700.10718089901435605No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAG290520.10711453313261342No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGG289210.10663153699326422No Hit
AAACATCACCTCTAGCATTACAAGTATTAGAGGC289190.10662416300640394No Hit
AAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG288100.10622228072251798No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC276040.1017757666457614No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA274770.10130751848013281No Hit
TGCTGCAGTTAAAAAGCTCGTAGTTGGATCT273600.10087614024880569No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCT271570.10012768058248597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)