FastQCFastQC Report
Thu 22 Jun 2023
ERR1719519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1719519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23069695
Total Bases740.6 Mbp
Sequences flagged as poor quality0
Sequence length1-51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC304992413.22047820744921No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC16793257.27935501531338No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC14900646.458967056131431No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC13994806.0663134037966255No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC7339043.181247086274873No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT6491672.8139383723972076No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3249541.4085751892255185No Hit
GCCGCCTGGATACCGCAGCTAGGAATAA2746111.1903538386614994No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC2659131.1526506960755225No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA2384621.0336590925887836No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2304800.999059588780866No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2130280.9234105609111868No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1979890.8582211424988497No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCAC1757340.7617525936081946No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1477850.640602313988113No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1461820.633653804265726No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC1414610.6131897279092766No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1250390.5420054318013308No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1245400.539842420976957No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT1192840.5170592849190248No Hit
TGACTCTAGATAACCTCGGGCCGATCGCAC1132320.4908257348005684No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCC1089790.4723902938465376No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC1057570.45842391934527094No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCAC1050500.45535929278648896No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA1001460.4341019679714014No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT982370.42582704279358696No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT935900.40568373357341747No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC924880.4009069040574659No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA914410.39636848254820883No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG914040.3962080989800689No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG898840.38961936861323915No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC895180.388032871695963No Hit
CTGGATACCGCAGCTAGGAATAATGGAAT872250.3780934251623179No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA831610.36047724081311No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT821100.3559214805397297No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT815380.353442037270107No Hit
ACTCTAGATAACCTCGGGCCGATCGCAC811520.3517688465322147No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT811100.3515867895089207No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT748580.3244863011843026No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAAT710470.3079667936658894No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC656590.2846114783918903No Hit
TGGATACCGCAGCTAGGAATAATGGAAT650730.28207134944783624No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT600000.260081461848542No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA577530.2503414111023141No Hit
CTCTAGATAACCTCGGGCCGATCGCAC550290.23853371273439028No Hit
GACTCTAGATAACCTCGGGCCGATCGCAC549770.2383083088007882No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT549390.23814359054161746No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCAT512580.2221875928572094No Hit
AACGATGCCGACTGGCGATGCGGCGGCG498050.21588928678944388No Hit
GATACCGCAGCTAGGAATAATGGAAT493240.21380430040362475No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG474320.20560306497333408No Hit
AAGTCCTTCTGATCGAGGCC467770.20276384234815414No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAAT467300.20256011186970616No Hit
GGATACCGCAGCTAGGAATAATGGAAT448240.1942981907649841No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG433580.18794353371381806No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAG431310.18695955884982443No Hit
GGGCCCAAGTCCTTCTGATCGAGGCC411970.17857626639623975No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT387300.16788258362323386No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC384790.16679457617450077No Hit
CCTCCGGGGCTACGCCTGTCTGAGCGTCGCT366730.15896612417285969No Hit
TGGATACCGCAGCTAGGAATAA349990.15170985138728535No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACT347620.15068252961298362No Hit
CCGCCTGGATACCGCAGCTAGGAATAA345190.14962919969249702No Hit
CCTGGATACCGCAGCTAGGAATAATGGAAT308110.1335561653502571No Hit
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCC304030.13178761140968703No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATA300290.13016643696416447No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT288890.12522488918904215No Hit
TTGGTGACTGTAGATAACCTCGGGCCGATCGCAC288040.1248564404514234No Hit
TCTAGATAACCTCGGGCCGATCGCAC283620.1229405070158058No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCC274160.11883988930066047No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA272420.11808565306129967No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT263680.1142971331003726No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT257990.11183069390384225No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT245680.10649468924491634No Hit
CTGGATACCGCAGCTAGGAATAATGGAATA244660.10605255075977381No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG241400.10463944148373006No Hit
CGCCTGGATACCGCAGCTAGGAATAA237130.10278852841357461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)