FastQCFastQC Report
Thu 22 Jun 2023
ERR1719520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1719520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29010875
Total Bases922.6 Mbp
Sequences flagged as poor quality0
Sequence length1-51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC376906412.991900451123934No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC21465827.3992321844825435No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC17298165.962646766083409No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC15563475.364702029842258No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC9234533.183127017023789No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT8427772.9050381968830656No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4154331.4319905897357457No Hit
GCCGCCTGGATACCGCAGCTAGGAATAA3765151.2978408958709449No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC3355431.1566110984242979No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3036221.046579946313236No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2814110.970019001495129No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA2694940.9289413021840948No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2653530.9146673445733712No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2412220.8314881919280269No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCAC2396420.8260419584035297No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG2220090.7652613028734914No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1870510.6447616626523675No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC1758490.6061485563603304No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1595600.5500006463093582No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT1428010.4922326541340101No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1371150.4726331074123066No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC1347250.4643948174606936No Hit
TGACTCTAGATAACCTCGGGCCGATCGCAC1276810.4401142674945171No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG1258910.4339441674889158No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1257750.43354431743268684No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA1250470.4310349136315261No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCC1249200.43059714675962035No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCAC1220340.4206491531193044No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1195600.4121213165752498No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1173970.4046654918198779No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG1171380.4037727231598495No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT1154900.3980920947748043No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT1035740.3570178424470134No Hit
CTGGATACCGCAGCTAGGAATAATGGAAT993670.34251638394222855No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA992040.34195452567356205No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT943010.32505396683140375No Hit
ACTCTAGATAACCTCGGGCCGATCGCAC942390.32484025387031584No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG939770.3239371442605575No Hit
AACGATGCCGACTGGCGATGCGGCGGCG914300.3151576779397381No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT813160.28029488941646885No Hit
GGGCCCAAGTCCTTCTGATCGAGGCC808050.2785334809791156No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC786220.2710087165588766No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAAT776490.2676548018630944No Hit
AAGTCCTTCTGATCGAGGCC770180.26547975543653884No Hit
GATACCGCAGCTAGGAATAATGGAAT766220.2641147500721712No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT762370.2627876615234804No Hit
TGGATACCGCAGCTAGGAATAATGGAAT755240.2603299624709699No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT750050.2585409781676699No Hit
CTCTAGATAACCTCGGGCCGATCGCAC734590.2532119420734466No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA719720.24808627799058114No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT695430.2397135556924774No Hit
TCTAGATAACCTCGGGCCGATCGCAC642570.22149280226811496No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCAT635710.21912817176317503No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC598420.20627437124871276No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG585470.20181052794857102No Hit
GACTCTAGATAACCTCGGGCCGATCGCAC582680.20084881962367562No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAAT544900.18782611693028908No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT544540.18770202553352838No Hit
TGGATACCGCAGCTAGGAATAA539320.18590270028049827No Hit
GGATACCGCAGCTAGGAATAATGGAAT538140.18549595625778265No Hit
CCGCCTGGATACCGCAGCTAGGAATAA471770.1626183284716507No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT444190.15311154868648394No Hit
CCTCCGGGGCTACGCCTGTCTGAGCGTCGCT433110.14929229125284915No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG423220.14588322482517332No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT400550.13806891381249273No Hit
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCC366060.12618026860616924No Hit
GGATACCGCAGCTAGGAATAA362140.12482905117477498No Hit
TTGGTGACTGTAGATAACCTCGGGCCGATCGCAC359230.12382597905095934No Hit
CCTGGATACCGCAGCTAGGAATAATGGAAT356050.12272983837957317No Hit
AACCTCGGGCCGATCGCAC352660.12156131106007662No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA349510.12047551133842051No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATA348440.12010668413138176No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACT345690.11915876373945976No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC342110.1179247437383395No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGT332020.11444673764579663No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG327750.11297487580088503No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCG315050.10859720708182707No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG310810.10713568618664553No Hit
CGCCTGGATACCGCAGCTAGGAATAA301320.10386449908870381No Hit
AGCTAGGAATAATGGAAT292400.10078979003563318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)