FastQCFastQC Report
Thu 22 Jun 2023
ERR1719521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1719521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30202884
Total Bases976.3 Mbp
Sequences flagged as poor quality0
Sequence length1-51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC382171312.653470443418582No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC22073837.308517292587026No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC18532236.135914040526726No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC11278073.734103670364724No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC10601663.5101482361750618No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC10021833.3181698807305953No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT8416112.7865252867904933No Hit
GCCGCCTGGATACCGCAGCTAGGAATAA4226491.3993663651457922No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC4045241.3393555396895211No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3485181.1539229167651672No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCC3150751.0431950803108736No Hit
AACGATGCCGACTGGCGATGCGGCGGCG2877960.9528758909248534No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2579390.8540210928201427No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2279150.7546133673857105No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2244590.7431707515083659No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1969060.6519443639885516No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1788910.5922977421626359No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC1780340.5894602647879587No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG1687930.5588638488960194No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG1632610.540547717231242No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1553260.5142753917142483No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA1541070.5102393533014926No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1321180.43743504759346824No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1220190.4039978433847575No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGC1136300.37622235015702477No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT1104040.36554125096133205No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1088340.3603430718735337No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1063620.3521584230168218No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA986530.3266343704131036No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC953150.3155824457028673No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC940440.3113742383012165No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT915450.30310019400796295No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC911270.3017162202126128No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA889240.2944222147792244No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT831810.2754074743325836No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT777140.2573065539039252No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAG762500.25245933467810555No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG745370.2467876908708453No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGC654420.21667467252465028No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC606370.20076559576231198No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCAC603480.1998087334971058No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG583530.19320340401929828No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT567520.1879025857265816No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGC565330.18717748940796516No Hit
ACGATGCCGACTGGCGATGCGGCGGCG542290.1795490788230687No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCC526550.17433765596689377No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT525150.1738741240737143No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT503570.16672911103456214No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACT500200.16561332354883723No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCC499350.16533189347083543No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC495760.16414326525903952No Hit
CCGCCTGGATACCGCAGCTAGGAATAA495000.16389163365988493No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA473710.1568426379414628No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC459560.152157654878256No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT454720.15055515890469268No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCG416710.13797026800487No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC403930.13373888400856024No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA393090.13014982277851347No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG390640.1293386419654494No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC389610.12899761492975306No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG383850.12709051228352897No Hit
CTCGCCCGTCACGTTGAACGCACGTTCG379630.12569329471980226No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATG374930.12413715193555688No Hit
TTGGTGACTGTAGATAACCTCGGGCCGATCGCAC372580.1233590805434342No Hit
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCC371060.12285581734512506No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCC370150.1225545216145584No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGAT341020.11290974729433123No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCC330260.10934717360103756No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC311200.10303651796960847No Hit
CGCTGAGAAGACGGTCGAACTTGACTATC308970.10229817788261544No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCT304830.10092744785564185No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT302460.1001427545793309No Hit

[OK]Adapter Content

Adapter graph