FastQCFastQC Report
Thu 22 Jun 2023
ERR1719522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1719522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19815295
Total Bases629.6 Mbp
Sequences flagged as poor quality0
Sequence length1-51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC236208411.92050887963061No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC14893267.516042531791729No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC11202515.653466173478618No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC8505814.292547751623178No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC6395633.22762290442812No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT5940162.9977651102342913No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2570541.297250432052614No Hit
GCCGCCTGGATACCGCAGCTAGGAATAA2186401.103390083266487No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2059421.0393082717163686No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC2037061.028024059192659No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1953470.9858394740022796No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1891570.9546009786884323No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1846060.9316338717137443No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1381680.6972795509731246No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1275760.6438258930790584No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCAC1245880.6287466323362837No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG1079520.5447912837028165No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC1077600.543822335221353No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT1046950.528354485764658No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1030440.5200225381454073No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT986890.49804456607888No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC882680.44545387792611724No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCC865470.4367686678396663No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC848150.4280279450797982No Hit
TGACTCTAGATAACCTCGGGCCGATCGCAC839610.4237181429799557No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT834170.4209727889491426No Hit
CTGGATACCGCAGCTAGGAATAATGGAAT816660.41213618066246305No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA814140.4108644357805422No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC809750.4086489754505295No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCAC793930.40066524369180473No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT769030.3880991930728258No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC749910.3784500811115858No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT684210.34529387526150884No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT676110.341206123855335No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG671310.33878375265167643No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAAT646710.32636910023292615No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA636000.3209641844847629No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA634630.32027279937038533No Hit
TGGATACCGCAGCTAGGAATAATGGAAT625830.3158317854970113No Hit
ACTCTAGATAACCTCGGGCCGATCGCAC593620.2995766653991273No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT584620.29503471939226744No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC575430.29039688785859613No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG549460.2772908503254683No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT541700.27337468354622024No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG485340.24493200832992898No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA465060.23469748999447146No Hit
GATACCGCAGCTAGGAATAATGGAAT443230.2236807476244992No Hit
GGATACCGCAGCTAGGAATAATGGAAT428470.21623195617324903No Hit
AAGTCCTTCTGATCGAGGCC425040.21450097008396796No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAAT410790.20730955557310654No Hit
CCGCCTGGATACCGCAGCTAGGAATAA371170.1873148999295746No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC369290.18636613787480832No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACT361280.18232380592870306No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT360370.1818645647213428No Hit
CCTCCGGGGCTACGCCTGTCTGAGCGTCGCT359340.18134476423389106No Hit
GGGCCCAAGTCCTTCTGATCGAGGCC350890.17708038159411707No Hit
AACGATGCCGACTGGCGATGCGGCGGCG350840.17705514856074564No Hit
GACTCTAGATAACCTCGGGCCGATCGCAC339870.1715190210390509No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCG333030.1680671420738374No Hit
CTCTAGATAACCTCGGGCCGATCGCAC332350.16772397281998577No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT327730.16539244053646437No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCC315160.15904885594688345No Hit
CCTGGATACCGCAGCTAGGAATAATGGAAT313770.15834737761915732No Hit
TGGATACCGCAGCTAGGAATAA313340.15813037353216292No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA304230.1535329148518859No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA274040.1382972093022082No Hit
AGCTAGGAATAATGGAAT273350.1379489934416823No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT270350.13643501143939568No Hit
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCC268500.13550138920465227No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC252930.1276438226127847No Hit
TTGGTGACTGTAGATAACCTCGGGCCGATCGCAC250890.12661431485122981No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATA250780.12655880217781265No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT250560.1264477768309783No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC242550.12240544488487301No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT240190.12121444570974088No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG236930.11956925193392276No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCAT234080.11813096903175047No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTG231950.11705604181012697No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT226060.1140835904789709No Hit
TCTAGATAACCTCGGGCCGATCGCAC220910.11148458804171221No Hit
CGCCTGGATACCGCAGCTAGGAATAA218770.11040461421341444No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA213200.10759365429583562No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGT208440.10519146951887418No Hit
CTGGATACCGCAGCTAGGAATAATGGAA201990.10193640821395794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)