FastQCFastQC Report
Fri 23 Jun 2023
ERR1719534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1719534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47535077
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length1-49
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC858928518.069361705251893No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC36644877.709016648905396No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC36419467.661596929778824No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC26310835.535034686069825No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT22197364.6696800343880795No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC20136584.2361517579954695No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC13902262.9246318460786336No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC13192832.7753883726747723No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT10994892.3130056147800078No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC8543761.7973590323625646No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC7891861.6602182005511426No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT6464911.3600293526399463No Hit
GCCGCCTGGATACCGCAGCTAGGAATAA6372311.340549001319594No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG3360110.7068695818037699No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC3095290.6511591429629956No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2943480.6192227268297051No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2921040.6145020023844707No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC2879700.6058052667086244No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2629000.553065265887757No Hit
AACGATGCCGACTGGCGATGCGGCGGCG2510310.5280963361014436No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2356460.49573076320040466No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1992090.41907789483542857No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT1894250.3984951996606632No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT1744060.3668995844899967No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1731460.36424891033625545No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1718360.3614930507002229No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA1715520.3608955971608082No Hit
CCTCCGGGGCTACGCCTGTCTGAGCGTCGCT1410680.29676611231743666No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1355910.28524409458724553No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1314110.27645058826769126No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCC1312990.27621497278735874No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1197110.25183718541152256No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCC1111320.23378946035997797No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1100000.2314080610409025No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG1058270.222629280688869No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1021820.2149612590298318No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCC992230.2087363821878315No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG980530.20627504190221463No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA960830.2021307338999367No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCC954030.2007002113407747No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC940910.19794014428545054No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC837920.17627403864308455No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACT835590.17578387429560702No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGC826670.17390736529152986No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG795980.1674510803884887No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT780220.1641356339866663No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCC775100.1630585346480032No Hit
TTGGTGACTGTAGATAACCTCGGGCCGATCGCAC733190.15424188752234483No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGC719950.15145657595127068No Hit
CGACTGCATAATTTGTGGTAGTGGGGG715190.15045521015985733No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA695590.14633193925403762No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA687670.14466580121454312No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCG645610.13581759844419733No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT635470.13368443686332937No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG625570.13160176431396123No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTG623060.13107373319285884No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG587500.1235929416923002No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG583830.12282088025228192No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT532900.11210668702608811No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGT523340.1100955406046781No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGG508010.10687055371762626No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT505060.10624995937210746No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTC500300.10524859358069409No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCC490630.10321430635317999No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT489100.10289243877736856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)