FastQCFastQC Report
Thu 22 Jun 2023
ERR1719535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1719535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49746314
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length1-49
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1043124620.968882237184445No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC46537359.354934317344597No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC45155819.077217258750066No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC34408146.916721508250842No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC21115864.24470846221893No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT20629774.14699468989803No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC14979903.011258281367339No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC13297472.6730563394103934No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC8605481.7298728906829157No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC8256511.6597229696254479No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC8228471.6540863711027916No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT8113941.631063559804652No Hit
GCCGCCTGGATACCGCAGCTAGGAATAA5275771.0605348569142228No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5274001.060179051657978No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC3114500.6260765370475488No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2837270.5703477849635251No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2772700.5573679288077504No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2425880.4876502005756647No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2367320.47587847413177187No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG2208140.4438801234600015No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1923510.3866638239769885No Hit
AACGATGCCGACTGGCGATGCGGCGGCG1899810.38189965190184744No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCC1843240.37052795509633135No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1816020.36505619290707647No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1626930.32704533646452677No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1521200.30579150045167164No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1502490.30203041777125433No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA1411070.2836531767961743No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT1296470.2606162941037199No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1269030.255100307532333No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1229760.24720625532175106No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1133770.22791035331783577No Hit
AACGATGCCGACTGGCGATGCGGCGGCGT1076160.2163295957967861No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCC1054810.21203782053078346No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCC1048200.2107090788676323No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCC997370.20049123639592675No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG950260.19102118802209145No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC874320.17575573538976175No Hit
CCTCCGGGGCTACGCCTGTCTGAGCGTCGCT870900.1750682472675262No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA863710.17362291405148128No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG861480.1731746396325967No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCC836190.1680908458865917No Hit
TTGGTGACTGTAGATAACCTCGGGCCGATCGCAC786650.15813231911011538No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT739220.14859794436226972No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC730380.146820928280234No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGC694050.13951787463087215No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCC661120.1328982887053702No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG640840.12882160475246468No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACT609440.12250957930269969No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG594710.11954855589903605No Hit
GCCGCCTGGCTACCGCAGCTAGGAATAATGGAAT574190.11542362716562275No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG550260.11061322050916174No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT515120.10354938056315087No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA510870.102695045908326No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGC504090.10133213085898184No Hit

[OK]Adapter Content

Adapter graph