FastQCFastQC Report
Thu 22 Jun 2023
ERR1802077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1802077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12627212
Total Bases397 Mbp
Sequences flagged as poor quality0
Sequence length1-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGCGCCGCAAGGCGCAAGGTCGCCCGACAAC1391881.1022860786688304No Hit
GAGGACACTGCGATCCATTACGAAACAGCT1324171.048663790550123No Hit
TTCGCGCCGCAAGGCGCAAGGTCGCCCGACAA1274671.0094627380929377No Hit
GCAGTCGGGCGCGTGATGGATTCCCCCGGACACCGG946700.7497300274993404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG756240.5988970486913501No Hit
AGCGAAACGGGAAGAGCTCAGCACGCAGGGGCG670220.5307743308657525No Hit
GCTTAAATGCGCATTTAGTCGGGATTA615490.48743142983581805No Hit
CAGTACGATGTACACATTTTTGAGTGCCTATA552730.43772924696282917No Hit
CTCTAAGGTCGTAACCAAACCGAGCTGATAGTGT513650.4067802140329948No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGC495710.3925728022939664No Hit
ATCTTGCATGGAATAATGGAATATGACCT491350.38911994191591937No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCA437170.34621260813550925No Hit
CCGGTCGCTGGTTCAAGTTCAACTAGAATGTGGCT435070.34454953318278014No Hit
GCTTAAATGCGCATTTAGTCGGGATTATGGATT431730.34190445206748726No Hit
GCAGTCGGGCGCGTGATGGATTCCCCCGGA421460.3337712236081884No Hit
TGGAGTTGAAATCTGGATAATTTTGCTGATCGTAT390990.3096407979845432No Hit
GAATAATCTTGCATGGAATAATGGAAT390980.30963287858000643No Hit
CAGTCGGGCGCGTGATGGATTCCCCCGGACACCGG385500.30529304489383724No Hit
TGCAGGAACCTCGCGGAACCTGTGCAATTATTAGT349020.27640305714357216No Hit
CATAAGGCTCAGTATAACAGCTATAATTTACAAGATC345840.2738846865008681No Hit
TTGCTGTGTCGATATTCTCTTCTTGGACCT320560.25386443183182483No Hit
CGGTCGCTGGTTCAAGTTCAACTAGAATGTGGCT309220.24488382708708778No Hit
ATAAGGCTCAGTATAACAGCTATAATTTACAAGATC262550.20792396611381833No Hit
GCGAAACGGGAAGAGCTCAGCACGCAGGGGCG261400.20701323459208573No Hit
CAGTACGATGTACACATTTTTGAGTGCC259090.20518385214408374No Hit
TCGCTTAAATGCGCATTTAGTCGGGATTA244550.19366903794756912No Hit
TCGCTTAAATGCGCATTTAGTCGGGATTATGGATT236070.18695338290035837No Hit
CGCTGGAGTTGAAATCTGGATAATTTTGCTGA232150.18384897632193076No Hit
CATGAACACCGACACGTTGAACGCATATTGCACATCG231480.18331837621796482No Hit
AATCTTGCATGGAATAATGGAATATGACCT229820.18200375506485517No Hit
CGGCCGAGAATAATCTTGCA226610.17946162620854073No Hit
GACCGCTTGGGAACGTCGCGTGTCGTTGGCCT217930.17258758307059388No Hit
TGCCCGGTTCCGTGTACTGGAGAGTTCGTTATCTGCC216890.17176396499876614No Hit
CAGTACGATGTACACATTTTTGAGTGCCTA205530.16276752144495554No Hit
AGTCGGGCGCGTGATGGATTCCCCCGGACACCGG205260.1625536975224618No Hit
CAGTCGGGCGCGTGATGGATTCCCCCGGA204930.16229235717274723No Hit
GCAGTCGGGCGCGTGATGGATTCCCCCGG202440.16032042544308278No Hit
GGGTGCCA201600.15965519546199114Illumina Small RNA Adapter 2 (100% over 8bp)
CGCTTAAATGCGCATTTAGTCGGGATTATGGATT184630.14621596596303285No Hit
TCACGTTGGCATCTTATTGCATGCACGCAGA184410.14604173906322312No Hit
TTTCGATCCTCTCAGACTACCCTTGCTTGGAGGT182210.144299470065126No Hit
AAGATACTGGAACGCTGGTGGCGCTGCTC182150.1442519536379052No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGT180590.14301652653016358No Hit
CTTAAATGCGCATTTAGTCGGGATTATGGATT179500.14215331143565185No Hit
TCACGTTGGCATCTTATTGCATGCACGCAGAC173490.13739374930903198No Hit
ACCGCTTGGGAACGTCGCGTGTCGTTGGCCT168540.1334736440633134No Hit
ACGGCCGAGAATAATCTTGCA166990.13224613636010862No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGC164760.13048010914840108No Hit
CGCTTAAATGCGCATTTAGTCGGGATTA162090.1283656281370741No Hit
TGCTGTGTCGATATTCTCTTCTTGGACCT161860.1281834818327276No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCT161420.12783502803310817No Hit
GGAGTTGAAATCTGGATAATTTTGCTGAT159670.1264491322391673No Hit
CATGTGGCAAAAAACCTACAAGACCTGCTAGCGTGAT158090.12519786632235208No Hit
GGAGTTGAAATCTGGATAATTTTGCTGA157590.12480189609551183No Hit
TTGCGGTTAAAACGTTCGAAGTTTATTCTTGT155980.1235268719650862No Hit
ATTCTCTTCTTGGACCTTGAAAATCGAAGAC155880.12344767791971814No Hit
TTCGCGCCGCAAGGCGCAAGGTCGCCCGACAACTGCCC153340.12143614916736965No Hit
GCAGTCGGGCGCGTGATGGATTCCCCCGGAC152800.12100850132238217No Hit
CGACACGTTGAACGCATATTGCACATCGTACC150500.119187038278917No Hit
ATGAACACCGACACGTTGAACGCATATTGCACATCGT144430.1143799597250763No Hit
AGGGAATCCGGTTACAATTCCGGAGCCTGTTGGGT141230.11184575027329866No Hit
AGTCGGGCGCGTGATGGATTCCCCCGGACACCGGG140470.11124387552850146No Hit
AGTACGATGTACACATTTTTGAGTGCCTATA140190.11102213220147092No Hit
TAATCTTGCATGGAATAATGGAATATGACCT139040.11011140067973832No Hit
CTAAGGTCGTAACCAAACCGAGCTGATAGTGT138210.10945409010318351No Hit
AGAGAGATATGGTTGGACAGGCTGGTAGAGCA136180.10784645098221206No Hit
ATCATGTGGCAAAAAACCTACAAGACCTGCTAGCGTG133690.10587451925254758No Hit
TGGAGTTGAAATCTGGATAATTTTGCTGATCGTA132950.10528848331682401No Hit
TGGAATGAGTTGAGCATAAATCCTTCAACA130310.10319776051910746No Hit
GCTGGAGTTGAAATCTGGATAATTTTGCTGA129260.10236622304274294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)