FastQCFastQC Report
Thu 22 Jun 2023
ERR1802078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1802078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17629538
Total Bases542.4 Mbp
Sequences flagged as poor quality0
Sequence length1-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACACTGCGATCCATTACGAAACAGCT15188868.615574611200817No Hit
CAGTACGATGTACACATTTTTGAGTGCCTATA6057543.436017438460384No Hit
ATCTTGCATGGAATAATGGAATATGACCT2176081.2343375078802405No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGC1915981.0868010267767652No Hit
CAGTACGATGTACACATTTTTGAGTGCC1872441.0621038395901243No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCGCA1863221.0568739804752683No Hit
CAGTACGATGTACACATTTTTGAGTGCCTA1679610.9527249097508964No Hit
CCGGTCGCTGGTTCAAGTTCAACTAGAATGTGGCT1635820.9278859151045251No Hit
CGGTCGCTGGTTCAAGTTCAACTAGAATGTGGCT1493180.8469762508807661No Hit
TTCGCGCCGCAAGGCGCAAGGTCGCCCGACAAC1415190.8027379957432804No Hit
TTCGCGCCGCAAGGCGCAAGGTCGCCCGACAA889290.5044318234544773No Hit
GAATAATCTTGCATGGAATAATGGAAT886920.5030874887362335No Hit
AATCTTGCATGGAATAATGGAATATGACCT872320.49480593308797993No Hit
GCAGTCGGGCGCGTGATGGATTCCCCCGGACACCGG871550.4943691661120104No Hit
TTTCGATCCTCTCAGACTACCCTTGCTTGGAGGT832300.4721053949343426No Hit
AGCGAAACGGGAAGAGCTCAGCACGCAGGGGCG780270.4425924264152583No Hit
TGGAGTTGAAATCTGGATAATTTTGCTGATCGTAT749850.42533729471526704No Hit
AGTACGATGTACACATTTTTGAGTGCCTATA671740.3810309719971107No Hit
GCAGTCGGGCGCGTGATGGATTCCCCCGGA648900.3680754424761443No Hit
CCAGTACGATGTACACATTTTTGAGTGCCTATA634490.3599016604972859No Hit
CTCTAAGGTCGTAACCAAACCGAGCTGATAGTGT622990.3533785173496889No Hit
GCTTAAATGCGCATTTAGTCGGGATTA569420.3229920148786656No Hit
TGCCCGGTTCCGTGTACTGGAGAGTTCGTTATCTGCCG551710.31294637443136625No Hit
ATTTCGATCCTCTCAGACTACCCTTGCT549980.3119650668100321No Hit
TAATCTTGCATGGAATAATGGAATATGACCT549490.3116871241889606No Hit
AGGACACTGCGATCCATTACGAAACAGCT524760.2976595302724325No Hit
CAGTACGATGTACACATTTTTGAG524140.29730784777230124No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG513640.2913519344636258No Hit
AGAGGACACTGCGATCCATTACGAAACAGCT503630.2856739637760218No Hit
CGCTGGAGTTGAAATCTGGATAATTTTGCTGA498670.2828605037749713No Hit
TTTCGATCCTCTCAGACTACCCTTGCTTGGAGG471010.26717092643040335No Hit
CGGCCGAGAATAATCTTGCA456980.25921269179033507No Hit
GAGGACACTGCGATCCATTACGAAACAGCTT451080.2558660357406984No Hit
TGCCCGGTTCCGTGTACTGGAGAGTTCGTTATCTGCC447730.2539658157803114No Hit
CGCATAAGGCTCAGTATAACAGCTATAATTTACAAGATC444920.25237189993294207No Hit
TTGCTGTGTCGATATTCTCTTCTTGGACCT440900.2500916359804778No Hit
CGACACGTTGAACGCATATTGCACATCGTACC426550.24195188779195465No Hit
TTTCGATCCTCTCAGACTACCCTTGCT423640.24030124896069313No Hit
CAGTCGGGCGCGTGATGGATTCCCCCGGACACCGG410800.23301801782894138No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGGCG406410.23052787883607612No Hit
ATTTCGATCCTCTCAGACTACCCTTGCTTGGAGG404150.22924593940011362No Hit
ATTTCGATCCTCTCAGACTACCCTTGCTTGGAGGT389350.22085093778407577No Hit
CATTTCGATCCTCTCAGACTACCCTTGCT387600.21985828556596324No Hit
ATCTTGCATGGAATAATGGAATATGACCTC386000.21895071782368886No Hit
GCGAAACGGGAAGAGCTCAGCACGCAGGGGCG375340.21290404774078595No Hit
ACGGCCGAGAATAATCTTGCA364170.2065680904400331No Hit
AGTACGATGTACACATTTTTGAGTGCCTA350450.19878569705003046No Hit
CATAAGGCTCAGTATAACAGCTATAATTTACAAGATC339370.19250079043478055No Hit
ATAATCTTGCATGGAATAATGGAAT324560.18410011652035352No Hit
TCGCTTAAATGCGCATTTAGTCGGGATTA321330.18226796414063717No Hit
CATGTGGCAAAAAACCTACAAGACCTGCTAGCGTGAT318670.18075913276910605No Hit
GCTTAAATGCGCATTTAGTCGGGATTATGGATT316990.17980618663971795No Hit
GCAGTCGGGCGCGTGATGGATTCCCC304710.17284060421776226No Hit
TGGAGTTGAAATCTGGATAATTTTGCTGATCGTA304660.1728122427258162No Hit
GGAGTTGAAATCTGGATAATTTTGCTGA302610.17164942155602717No Hit
CATGAACACCGACACGTTGAACGCATATTGCACATCG300200.1702823976442264No Hit
GCAGTCGGGCGCGTGATGGATTCCCCCGG290880.16499581554547826No Hit
CGGTCGCTGGTTCAAGTTCAAC289180.16403152481931177No Hit
GCAGTCGGGCGCGTGATGGATTCCCCCGGAC280250.15896616235774302No Hit
CATGAACACCGACACGTTGAACGCATATTGCACATCGT277450.15737791880876287No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCG276180.15665753691333262No Hit
AAGTTCAACTAGAATGTGGCT275080.15603358409051898No Hit
CGACACGTTGAACGCATATTGCACATCGTACCT274000.15542097586448378No Hit
CATTTCGATCCTCTCAGACTACCCTTGCCT271300.15388945529939582No Hit
CAGTCGGGCGCGTGATGGATTCCCCCGGA267410.1516829312259913No Hit
CCGGTCGCTGGTTCAAGTTCAAC267290.1516148636453207No Hit
AATCTTGCATGGAATAATGGAATATGACC262850.14909636316050937No Hit
GCTGGAGTTGAAATCTGGATAATTTTGCTGA257150.14586315307865697No Hit
GGAGTTGAAATCTGGATAATTTTGCTGAT255790.14509172049772376No Hit
CAGTACGATGTACACATTTTTGAGTGCCT250940.14234065577895463No Hit
TCGGGTGCCA247130.1401795100926638Illumina Small RNA Adapter 2 (100% over 10bp)
TGCAGGAACCTCGCGGAACCTGTGCAATTATTAGT246340.13973139851991584No Hit
ATAATCTTGCATGGAATAATGGAATATGACCT244820.1388692091647552No Hit
ATCATGTGGCAAAAAACCTACAAGACCTGCTAGCGTG233810.1326240086382298No Hit
GAGGACACTGCGATCCATTACGAAACAGC233430.1324084612994396No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGC229150.1299807175888557No Hit
GCATAAGGCTCAGTATAACAGCTATAATTTACAAGATC226210.12831306186242658No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGCGG221940.1258909904502319No Hit
ATAAGGCTCAGTATAACAGCTATAATTTACAAGATC220310.12496640581278987No Hit
CTAAGGTCGTAACCAAACCGAGCTGATAGTGT215000.12195441536811684No Hit
TAATCTTGCATGGAATAATGGAATATGACC213760.12125105036785422No Hit
TCACGTTGGCATCTTATTGCATGCACGCAGA213290.12098445234356113No Hit
TGGAGTTGAAATCTGGATAATTTTGCTGAT211130.11975923589149075No Hit
ACCGCTTGGGAACGTCGCGTGTCGTTGGCCT209380.11876658367337817No Hit
CGGTCGCTGGTTCAAGTTCAACTAGAATG206680.11723506310829018No Hit
TGCTGTGTCGATATTCTCTTCTTGGACCT206000.11684934681782358No Hit
TGCCCGGTTCCGTGTACTGGAGAGTTCGTTATC204980.11627077238212369No Hit
TCACGTTGGCATCTTATTGCATGCACGCAGAC204720.11612329262400409No Hit
ATGAACACCGACACGTTGAACGCATATTGCACATCGT204030.11573190403514827No Hit
AGAGAGATATGGTTGGACAGGCTGGTAGAGCA204010.11572055943836987No Hit
GCAGTCGGGCGCGTGA200500.11372958270375547No Hit
ATTTCGATCCTCTCAGACTACCCTTGCCT195090.11066086927519032No Hit
TGGAGTTGAAATCTGGATAATTTTGCTGATCGT192970.10945834201667679No Hit
GACCGCTTGGGAACGTCGCGTGTCGTTGGCCT187840.10654845294300963No Hit
GGGTGCCA182740.10365558076451012Illumina Small RNA Adapter 2 (100% over 8bp)
CAGTACGATGTACACATTTTTGAGTGC182580.10356482399028266No Hit
TGTTGCCTTACTTACATGATCAGGTGGAA180610.10244738120760737No Hit
TCGCTTAAATGCGCATTTAGTCGGGATTATGGATT180300.10227153995754171No Hit
CTCCCAGAGAGGGTGATAGGCCCGTAGAGC179610.1018801513686859No Hit
TGCCCGGTTCCGTGTACTGGAGAGTTCGTTATCTGC176930.10035997540037635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)