FastQCFastQC Report
Thu 22 Jun 2023
ERR1994966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1994966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32968604
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGACAACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG469590.14243551228314064RNA PCR Primer, Index 21 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)