FastQCFastQC Report
Fri 23 Jun 2023
ERR1994974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1994974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31871091
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGACAACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGG744590.23362551347865687RNA PCR Primer, Index 23 (100% over 39bp)
GGCGGACAACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGG375660.11786857249411387RNA PCR Primer, Index 23 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)