FastQCFastQC Report
Thu 22 Jun 2023
ERR2093966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2093966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5129748
Total Bases512.9 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCAT1228772.3953808257247724No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA937691.8279455443035406Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCA359030.6998979287091686No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCAT306770.5980215792276735No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGTAGGCACCATC283650.5529511391202843No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACTGTAGGCACCATCAA259990.5068280157231896No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCTGTAGGCACCATCA186680.36391651207817616No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGCTGTAGGCACCATCAAT176320.343720588223827No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGTAGGCACC167900.32730652655841963No Hit
TCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCTGTAGGCACCATCAAT153200.2986501481164377No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCATC132120.2575565115479357No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA116410.22693122547150463No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCA116130.22638538969165736No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA112590.21948446590358822Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGAATACAAGCTTGGGCTGCAGGTCGACCCGTCTGTAGGCACCATCAATA106500.20761253769191No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCTGTAGGCACCATCAATA106160.2069497371020955No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCCTGTAGGCACCATCAA103370.20151087343861723No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCTGTAGGCACCATCAATA102600.20000982504403725No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCTGTAGGCACCATCAATA100710.19632543353006815No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGTAGGCACCATCAAT95870.1868902721927081No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATAGATCGGAA95220.18562315341806265No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGTAGGCACC91340.178059429040179No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCCGCTGTAGGCACCATCAATAG88970.17343931904647167No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCCTGTAGGCACCATCAATAG85700.1670647369032553No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA78830.1536722661620025Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAATACAAGCTTGGGCTGCAGGTCGACCCCTGTAGGCACCATCAATAGA77570.15121600515268976No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG71620.13961699483093518No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG70470.13737516930656243No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA66170.12899269125890783No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA65950.12856382028902782No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGTAGGCACCATC65120.12694580708448058No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAG64790.12630250062966056No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCCTGTAGGCACCATCAATA64590.12591261792976965No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCCTGTAGGCACCATCAAT64410.12556172349986783No Hit
ACAAAGCACCCAACTTACACTTAGGAGACTGTAGGCACCATCAATAGATC63180.12316394489553872No Hit
CCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCTGTAGGCACCATCAAT63080.12296900354559327No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCCTGTAGGCACCATCAATAGAT62020.12090262523617144No Hit
TCCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCA61470.11983044781147144No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACTGTAGGCACCATCAA60600.11813445806694597No Hit
CGCCGGGTCCGCCCCCGGGGCCTGTAGGCACCATCAATAGATCGGAAGAG53060.10343588028105864No Hit
ACAAAGCACCCAACTTACACTTAGGAGCTGTAGGCACCATCAATAGATCG51400.10019985387196408No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)