FastQCFastQC Report
Fri 23 Jun 2023
ERR2098884.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2098884.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281546867
Total Bases14.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC48945301.7384423602909422TruSeq Adapter, Index 1 (100% over 36bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT45776591.625895911674272No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA34711811.2328963333838128No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG32207391.1439441803484889No Hit
ACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACT26305700.9343275696973037Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC24653540.875646042973016No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG17665400.6274408303041071No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA17561010.6237330994700786No Hit
ACCCGAAGATCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA16775960.5958496423261567No Hit
ATTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA13725630.48750782227670825Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA13083120.4646871101570454Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG12310750.43725402208080694No Hit
AGGCTTGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC12192660.4330596937525148TruSeq Adapter, Index 1 (100% over 36bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT11008250.39099174206047904Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCAGATCGGAAGAGCACAC10945780.388772928522767No Hit
AGGCTTGTAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC10923990.3879989898804308TruSeq Adapter, Index 1 (100% over 36bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA10674100.37912338054893Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACAGATCGGAAGAGCACACG10238720.36365952528962076No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCAGATCGGAAGAGCACACG10022980.3559968578872519No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC9710090.3448836104434435No Hit
AACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAAC9457070.3358968295676329Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCAGATCGGAAGAGCAC8789900.3122002419582953No Hit
AGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC8381650.2976999918098894TruSeq Adapter, Index 1 (100% over 36bp)
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAGATCGGAAGAGCAC7749500.2752472468464726No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGAGATCGGAAGAGCACACG7677200.2726792907271101No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGCAGATCGGAAGAGCACACGT7527020.26734518768415205No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT7265130.25804336156935465No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCAGATCGGAAGAGCACAC7229530.25677891844557443No Hit
CGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACTC7194710.25554217941270857Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGGCGTAGTTCAATTGGTAGAGCACCGGAGATCGGAAGAGCACACGTC7121890.25295575389940317No Hit
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAGATCGGAAGAG6798650.24147489448000142No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCAGATCGGAAGAGCACACGTCTG6582890.23381151671632702Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCAGATCGGAAGAGCACACGT6462250.22952661732158433No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGT6430060.228383290800391No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCAGATCGGAAGAGCACACGT6209300.2205423227103429No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGAGATCGGAAGAGCAC5233940.18589942256398825No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT5165740.18347709051225208No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCAGATCGGAAGAGCACACGT5158900.18323414694577297No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGAGATCGGAAGAGCAC5130110.18221158184651368No Hit
AGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCAGATCGGAAGAGCACA4990840.1772649808957029No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT4688990.1665438528925275No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC4687040.16647459266524178No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTAGATCGGAAGAGCACA4488650.15942816369538929No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCAGATCGGAAGAGCACACG4285930.15222794150289728No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGAGATCGGAAGAGCAC4270440.1516777666717918No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCAGATCGGAAGA4268070.1515935888570907No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCAGATCGGAAGAGCACACGTCTG4200420.14919079174125563Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATGTCGACTTGGAGGTTGTGCCAGATCGGAAGAGCACACGTCTGAAC4121080.14637278844235904Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCAGATCGGAAGAGCACACG4026400.1430099380221482No Hit
AGGCTTGTAGCTCAGATGGAAGAGCACACGTCTGAACTCCAGTCACATCA3967970.1409346174681461TruSeq Adapter, Index 1 (100% over 33bp)
AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCAGATCGGAAGAGCACA3962810.1407513442513285No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC3870740.13748119598148467Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCAGATCGGAAGAGCAC3853400.13686531272962096No Hit
AAGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACT3725930.1323378249490519Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTAGATCGGAAGAGCACAC3660890.13002772998358386No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCAGATCGGAAGAGCACACGT3626720.12881407769314654No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCAGATCGGAAG3618990.12853952304857294No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3589130.12747895361946968Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACGGAGATCGGAAGAGCACA3509100.12463644285553353No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGG3503240.12442830699302367No Hit
ATTCATCTCCGGGGGTAGAGCACGACCAGATCGGAAGAGCACACGTCTGA3465800.123098510629138Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCGTAGTTCAATTGGTAGAGCACCGGTAGATCGGAAGAGCACACGT3375680.11989762258657988No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACGACCAGATCGGAAGAGCACACGT3298980.11717338698000856No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC3131720.11123263538215823No Hit
AGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCT3131620.11122908357563076No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGCAGATCGGAAGAGCACACGTC2934730.10423593170369037No Hit
ACTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA2928520.10401536451833435Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)