FastQCFastQC Report
Fri 23 Jun 2023
ERR2098885.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2098885.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65296139
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC11761311.8012259499753884TruSeq Adapter, Index 1 (100% over 36bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT11131661.7047960523362646No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA8219601.2588186875796745No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG7685271.1769868965759216No Hit
ACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACT6195530.9488355812278578Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC5786520.8861963492205872No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA4206870.644275460146273No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG4189100.6415540128643747No Hit
ACCCGAAGATCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA4019990.6156550849047904No Hit
ATTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA3319100.5083148943921477Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA3094590.47393154440571134Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG2968230.45457971106071066No Hit
AGGCTTGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2944970.45101747899672906TruSeq Adapter, Index 1 (100% over 36bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCAGATCGGAAGAGCACAC2717580.416193061583626No Hit
AGGCTTGTAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC2591050.3968151930085789TruSeq Adapter, Index 1 (100% over 36bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT2590710.39676312254848634Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA2520590.38602435589644896Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACAGATCGGAAGAGCACACG2457060.3762948372797356No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC2314850.3545156016039478No Hit
AACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAAC2221730.34025442147505847Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCAGATCGGAAGAGCACACG2204320.33758810762149355No Hit
AGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCAGATCGGAAGAGCAC2086140.3194890282869558No Hit
AGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1968770.30151399916616817TruSeq Adapter, Index 1 (100% over 36bp)
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAGATCGGAAGAGCAC1844610.2824990923276489No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGCAGATCGGAAGAGCACACGT1822930.27917883475468586No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGAGATCGGAAGAGCACACG1795350.27495500155070424No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT1738570.266259234715241No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACCGGAGATCGGAAGAGCACACGTC1697000.2598928552268611No Hit
CGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACTC1676340.2567288090341758Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAGATCGGAAGAG1609710.24652453034014768No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCAGATCGGAAGAGCACAC1594590.2442089263501476No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCAGATCGGAAGAGCACACGTCTG1559830.23888548754774Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGT1524810.23352223015820275No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCAGATCGGAAGAGCACACGT1506810.23076555874153598No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCAGATCGGAAGAGCACACGT1475260.22593372634176734No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCAGATCGGAAGAGCACACGT1236980.18944152272158082No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT1230980.18852263224935856No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGAGATCGGAAGAGCAC1230690.18847821920986782No Hit
AGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCAGATCGGAAGAGCACA1177210.1802878421341268No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT1165800.17854041875278415No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGAGATCGGAAGAGCAC1163700.17821880708750634No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC1109900.16997942251991346No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTAGATCGGAAGAGCACA1079980.16539722203176516No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGAGATCGGAAGAGCAC1063540.16287946213787619No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCAGATCGGAAGAGCACACG1021060.1563737175945426No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCAGATCGGAAGA1010530.15476106481579255No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCAGATCGGAAGAGCACACG1001810.15342561066282956No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCAGATCGGAAGAGCACACGTCTG995500.1524592441828758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATGTCGACTTGGAGGTTGTGCCAGATCGGAAGAGCACACGTCTGAAC976840.1496014948142646Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCTTGTAGCTCAGATGGAAGAGCACACGTCTGAACTCCAGTCACATCA970140.14857540045361642TruSeq Adapter, Index 1 (100% over 33bp)
AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCAGATCGGAAGAGCACA925890.14179858322097727No Hit
AGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCAGATCGGAAGAGCAC916980.14043403086972722No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC882140.13509834019435665Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTAGATCGGAAGAGCACAC881740.13503708082954186No Hit
AAGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACT866650.13272607129190286Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG846350.1296171585275509Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCAGATCGGAAG844260.1292970783463935No Hit
ATTCATCTCCGGGGGTAGAGCACGACCAGATCGGAAGAGCACACGTCTGA844250.12929554686227313Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGG842270.12899231300643976No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCAGATCGGAAGAGCACACGT826280.12654346989796747No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACGGAGATCGGAAGAGCACA823690.12614681551079154No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACCGGTAGATCGGAAGAGCACACGT811700.12431056605046739No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACGACCAGATCGGAAGAGCACACGT787300.12057374479676355No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC749080.11472041248870779No Hit
AGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCT740010.11333135639153183No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGCAGATCGGAAGAGCACACGTC708690.10853474812653165No Hit
ACTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA694170.10631103318375379Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)