FastQCFastQC Report
Thu 22 Jun 2023
ERR2098886.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2098886.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276916893
Total Bases14.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT40821431.4741401132216227No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG39541251.4279103586504562No Hit
ACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACT30192041.0902924582502809Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCTTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA28933901.044858610341262TruSeq Adapter, Index 2 (100% over 36bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC24231240.8750365402951419No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA18069540.6525257381101701No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA17857630.6448732616684385No Hit
ATTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA16318170.5892804091226027Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCGAAGATCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA14670970.5297968585831273No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG12147430.4386669902438924No Hit
AACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAAC11946530.4314121060140596Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCAGATCGGAAGAGCACACG11878210.4289449398090711No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCAGATCGGAAGAGCACAC11102000.4009145083106216No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCAGATCGGAAGAGCACAC10737720.3877596589963184No Hit
AGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCAGATCGGAAGAGCAC10545260.38080955935035715No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGAGATCGGAAGAGCACACG10128510.3657599177237627No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT10017940.36176702300354063Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACTC9844030.3554867994275813Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC9758930.3524136752466019No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA9741300.3517770221407186Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG9535340.34433941160823295No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCAGATCGGAAGAGCACACGTCTG8434400.304582357133409Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCAGATCGGAAGAGCACACGT8268520.2985921122551379No Hit
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAGATCGGAAGAG7640990.2759308006536098No Hit
AGGCTTGTAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA7608420.2747546354999729TruSeq Adapter, Index 2 (100% over 36bp)
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAGATCGGAAGAGCAC7549700.27263414370317957No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGAGATCGGAAGAGCAC7547860.27256769777494216No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACCGGAGATCGGAAGAGCACACGTC7398510.267174382893282No Hit
AGGCTTGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA7392990.2669750451085698TruSeq Adapter, Index 2 (100% over 36bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGCAGATCGGAAGAGCACACGT7358250.2657205170939138No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCAGATCGGAAGA7324350.2644963230899749No Hit
AGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCAGATCGGAAGAGCACA6747470.24366408011085114No Hit
ACGATGTCGACTTGGAGGTTGTGCCAGATCGGAAGAGCACACGTCTGAAC6681330.24127563788605705Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA6526620.2356887631264879Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACT6113930.220785735885026Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGAATAGCTCAGTTGGTAGAGCACGACCAGATCGGAAGAGCACACGT6043340.21823659562726644No Hit
AGGCTTGTAGCTCAGATGGAAGAGCACACGTCTGAACTCCAGTCACCGAT5961050.2152649459345191TruSeq Adapter, Index 2 (100% over 33bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGT5625770.2031573422283053No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT5389380.19462084604567623No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCAGATCGGAAGAGCACACG4999790.18055200409893374No Hit
AGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA4961040.17915266729502125TruSeq Adapter, Index 2 (100% over 36bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT4940170.1783990115763721No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC4831590.1744779795720155Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCAGATCGGAAGAGCACA4804470.17349862436886435No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCAGATCGGAAGAGCACACGT4763360.17201406343960388No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCAGATCGGAAGAGCACACGT4320410.15601828957397698No Hit
AGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCAGATCGGAAGAGCAC4293880.15506024040216282No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCAGATCGGAAG3932350.14200469886104058No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCAGATCGGAAGAGCACACG3689940.13325080893493918No Hit
AGGGGCGTAGTTCAATTGGTAGAGCACCGGTAGATCGGAAGAGCACACGT3602000.13007512690820203No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAGATCGGAAGAG3559460.12853892593688748No Hit
ACTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA3434980.12404371444395773Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAGATCGGAAGAGCAC3415400.12333664309890982No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACAGATCGGAAGAGCACACG3365100.12152021364763761No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC3349910.1209716736205039No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCAGATCGGAAGAGCACACGTCTG3168710.11442819416582144Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCAGATCGGAAGAG3128720.11298407858418373No Hit
AGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGT2998460.10828014020798653No Hit
ATTCATCTCCGGGGGTAGAGCACGACCAGATCGGAAGAGCACACGTCTGA2945070.10635212493157649Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTAGATCGGAAGAGCACACGT2927430.105715110706518No Hit
ACGATGTCGACTTGGAGGTTGCGCAGATCGGAAGAGCACACGTCTGAACT2906960.10497589975487699Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACTC2841380.10260768020389423Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)