FastQCFastQC Report
Mon 26 Jun 2023
ERR2098888.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2098888.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248954880
Total Bases12.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA40385321.6221943510406382No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG40308721.619117488277394No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCAGATCGGAAGAGCACACG25028911.0053592843811698No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT21336560.8570452605709113No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC20288470.8149456640496463No Hit
AGGCTTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA19216080.7718699870434353TruSeq Adapter, Index 3 (100% over 36bp)
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCAGATCGGAAGAGCACAC17968870.7217721540545821No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGAGATCGGAAGAGCAC15683910.6299900608495804No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG14373820.5773664689762257No Hit
AGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGT13842390.5560200306175963No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACAGATCGGAAGAGCACACG11438130.4594459044144867No Hit
ACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACT10505350.4219780708857766Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACT9921950.3985441056628414Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA9768480.3923795347976308Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAGATCGGAAGAG9538860.3831561767337117No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGAGATCGGAAGAGCAC8500870.34146227621647746No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCAGATCGGAAGAGCACAC7660480.3077055569266206No Hit
ATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGT7621770.30615065669731No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGAGATCGGAAGAGCAC7240880.2908510971948009No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC6870310.2759660706389849No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA5910420.23740928476678183No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCAGATCGGAAG5878040.23610864747861138No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGT5869560.23576802350691017No Hit
AGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCT5641720.22661616434271142No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGAGATCGGAAGAGCACACG5571190.2237831208610974No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG5054660.2030351845282165No Hit
GGAGCGGCAGTTCAGTCGGTTAGAATACCTGCCAGATCGGAAGAGCACAC4996420.20069580479804214No Hit
AACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAAC4904960.19702204672589668Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTG4762200.19128767429664364No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA4724240.1897629000082264Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCAGATCGGAAGAGCAC4689300.18835943284180653No Hit
AGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACTC4609300.185145999146512Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA4452500.17884766910373479TruSeq Adapter, Index 3 (100% over 36bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTAGATCGGAAGAGCACA4427710.17785190633740539No Hit
AGGCTTGTAGCTCAGATGGAAGAGCACACGTCTGAACTCCAGTCACTTAG4362930.17524982840264067TruSeq Adapter, Index 3 (100% over 33bp)
AAGGCGCTTGAGAGAACTCGGGTGAAGGAGATCGGAAGAGCACACGTCTG4194600.16848836222852914Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAGATCGGAAGAGCAC4173040.16762234184764727No Hit
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACAGATCGGAAGAGCACACGT3895050.15645606143571075No Hit
GGAGCGGCAGTTCAGTCGGTTAGAATACCTGCCTGAGATCGGAAGAGCAC3824920.1536390851225732No Hit
AGGCTTGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA3659990.14701418988051168TruSeq Adapter, Index 3 (100% over 36bp)
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCAGATCGGAAGAGC3569580.14338260812561698No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA3551440.14265396203520894No Hit
ACGATGTCGACTTGGAGGTTGTGCCAGATCGGAAGAGCACACGTCTGAAC3471370.13943771658543105Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCAGATCGGAAGAGCACACGT3456460.13883881288047056No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCAGATCGGAAGAGCACACG3292680.13226011074777888No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT3158170.12685712366835308No Hit
AGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGT3000830.12053710294813261No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACGGAGATCGGAAGAGCACA2979280.11967148424646265No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT2944340.11826801708004278Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT2895770.1163170611477871No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGG2846900.11435405483917406No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCGAGATCGGAAGAGCACA2709210.10882333377036033No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCAGATCGGAAGAGCACACGT2685990.10789063464030108No Hit
AGGCTTGTAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA2634540.10582399509501482TruSeq Adapter, Index 3 (100% over 36bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCAGATCGGAAGAGCACACG2544190.10219482341539159No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)