FastQCFastQC Report
Fri 23 Jun 2023
ERR2098889.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2098889.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80736378
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA14736131.8252156419501504No Hit
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG12809551.586589628779235No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCAGATCGGAAGAGCACACG8094491.002582751482857No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC7465810.9247145072571871No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT6901660.8548389426139478No Hit
AGGCTTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA6026620.7464565725254606TruSeq Adapter, Index 3 (100% over 36bp)
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCAGATCGGAAGAGCACAC5846860.724191516245626No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG5307960.6574434141695086No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGAGATCGGAAGAGCAC5121440.6343410649410108No Hit
AGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGT4633010.5738441722020278No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACAGATCGGAAGAGCACACG3758640.4655447882489849No Hit
ACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACT3299310.4086522187061698Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACT3296350.40828559339137055Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA3232970.4004353526981357Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAGATCGGAAGAG3138920.3887863287600046No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGAGATCGGAAGAGCAC2760910.3419660465818766No Hit
ATGCGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGT2573530.3187571778362413No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC2536100.31412110164268203No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCAGATCGGAAGAGCACAC2476610.30675267597463934No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGAGATCGGAAGAGCAC2398170.2970371051324596No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGT2170950.2688936578254724No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA2035130.25207100571194807No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCAGATCGGAAG1923900.23829406862913766No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGAGATCGGAAGAGCACACG1899930.23532514673868574No Hit
AGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCT1836070.22741545329169957No Hit
GGAGCGGCAGTTCAGTCGGTTAGAATACCTGCCAGATCGGAAGAGCACAC1642090.20338910918198486No Hit
AACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAAC1630420.20194366410640816Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG1629700.2018544849757813No Hit
AAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTG1604860.1987778049691553No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1578830.19555373167718768Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCAGATCGGAAGAGCAC1560030.19322516548859797No Hit
AGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACTC1534970.1901212363031693Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTAGATCGGAAGAGCACA1458760.1806818730461255No Hit
AGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA1433530.1775568876770766TruSeq Adapter, Index 3 (100% over 36bp)
AAGGCGCTTGAGAGAACTCGGGTGAAGGAGATCGGAAGAGCACACGTCTG1396460.17296540104883082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAGATCGGAAGAGCAC1390890.17227550138550926No Hit
AGGCTTGTAGCTCAGATGGAAGAGCACACGTCTGAACTCCAGTCACTTAG1360230.16847795673964963TruSeq Adapter, Index 3 (100% over 33bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA1317030.1631272089020392No Hit
AAGGCGCTTGAGAGAACTCGGGTGAAGGAACAGATCGGAAGAGCACACGT1307120.16189975725688363No Hit
GGAGCGGCAGTTCAGTCGGTTAGAATACCTGCCTGAGATCGGAAGAGCAC1267800.15702958584542895No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCAGATCGGAAGAGCACACGT1178460.14596394205348176No Hit
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCAGATCGGAAGAGC1171880.14514894388747537No Hit
AGGCTTGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA1149860.1424215488091378TruSeq Adapter, Index 3 (100% over 36bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT1101020.13637223111495042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATGTCGACTTGGAGGTTGTGCCAGATCGGAAGAGCACACGTCTGAAC1097040.13587926869842984Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACGGAGATCGGAAGAGCACA1089330.13492430884130074No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCAGATCGGAAGAGCACACG1062380.13158628443797665No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT1022680.12666904626313555No Hit
AGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGT1011420.1252743837480547No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCGAGATCGGAAGAGCACA1007830.12482972669395696No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT941970.11667231343967399No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGG935860.11591552942838232No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA871900.1079934499910313Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGAATAGCTCAGTTGGTAGAGCACGACCAGATCGGAAGAGCACACGT860430.10657277689618427No Hit
AGGCTTGTAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA838890.10390483457159794TruSeq Adapter, Index 3 (100% over 36bp)
AGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAG837480.10373019210745373No Hit
ACCCGAAGATCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA834680.10338338437723824No Hit
AGGCGCTTGAGAGAACTCGGGTGAAGGAGATCGGAAGAGCACACGTCTGA827100.10244452630758343Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCAGATCGGAAGAGCACACG818190.10134093456607626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)