FastQCFastQC Report
Fri 23 Jun 2023
ERR2098890.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2098890.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280597521
Total Bases14.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG40890041.4572487972906931No Hit
ACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACT38732671.3803639412765876Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCAGATCGGAAGAGCACACG36135391.2878014699210403No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA35372871.2606266040390286No Hit
AGGCTTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA27598010.9835443271788563TruSeq Adapter, Index 4 (100% over 36bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC25429150.9062499878607266No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGAGATCGGAAGAGCAC24161920.8610881491002196No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCAGATCGGAAGAGCACAC21147690.7536663162466072No Hit
ATTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA19923940.7100540278828764Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAAC18269090.6510780970156895Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG17375970.6192488778259734No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT15431850.5499638751263237No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCAGATCGGAAGAGCACAC12487730.44504063883016276No Hit
AAGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACT11729590.41802186841130357Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAGATCGGAAGAG11324690.4035919476280761No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGAGATCGGAAGAGCACACG11207850.39942797641465977No Hit
CGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACTC9293050.3311878867240598Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACAGATCGGAAGAGCACACG9261600.33006706427744953No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGAGATCGGAAGAGCAC8944290.31875869637494053No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA8482920.3023162845405181No Hit
ACGATGTCGACTTGGAGGTTGTGCCAGATCGGAAGAGCACACGTCTGAAC7848480.27970596361754746Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCAGATCGGAAG7741680.27589980026944No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAGATCGGAAGAGCAC7661230.27303270437660065No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA7457650.2657774727810229Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGT7085480.25251399138340924No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGT6486090.23115279054799634No Hit
AGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACTC6272170.22352905961703062Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGAGATCGGAAGAGCAC5944600.21185504343782138No Hit
AGGCTTGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA5637160.20089842490090995TruSeq Adapter, Index 4 (100% over 36bp)
AGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA5625850.2004953564789334TruSeq Adapter, Index 4 (100% over 36bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCAGATCGGAAGAGCACACGT5512490.19645540631843286No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG5424640.19332458749697937No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCAGATCGGAAGAGCACACG5397730.19236556263089724No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT4915240.17517047130291646No Hit
GGAGCGGCAGTTCAGTCGGTTAGAATACCTGCCAGATCGGAAGAGCACAC4847960.1727727309465432No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT4843850.1726262578064615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGCGGCAGTTCAGTCGGTTAGAATACCTGCCTGAGATCGGAAGAGCAC4751620.16933934352185526No Hit
AGGCTTGTAGCTCAGATGGAAGAGCACACGTCTGAACTCCAGTCACTGAC4576100.1630841207609956TruSeq Adapter, Index 4 (100% over 33bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA4405270.15699604131570358Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCAGATCGGAAGA4041630.14403655405066818No Hit
AGGCTTGTAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA3906010.13920329681030932TruSeq Adapter, Index 4 (100% over 36bp)
AGGGGCGTAGTTCAATTGGTAGAGCACCGGAGATCGGAAGAGCACACGTC3881310.13832303244047547No Hit
AGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCAGATCGGAAGAGCAC3797570.13533868675910363No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTCGAGATCGGAAGAGCACACGTCT3768480.1343019705437811Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA3712980.1323240485791747Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCAGATCGGAAGAGCACACGTCTG3711910.13228591566922646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATGTCGACTTGGAGGTTGTGCCCAGATCGGAAGAGCACACGTCTGAA3558840.12683077125260844Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCGAAGATCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA3538300.12609876193453612No Hit
AGAGAACTCGGGTGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC3319200.11829042495354047Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGAGAT3282350.11697715604550904No Hit
TGAGTCTCGTAGAGGGGGGTAGAGATCGGAAGAGCACACGTCTGAACTCC3235730.11531570159523968Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGCGCTTGAGAGAACTCGGGTGAAGGAGATCGGAAGAGCACACGTCTG3209490.11438055434566721Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGTCGACTTGGAGGTTGTGCCAGATCGGAAGAGCACACGTCTGAA3125340.11138159698852079Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCTCCTAAAGAGTAACGGAGGAGCACGAAGGTTGGCTAATCCTGGTC3088050.1100526472577069No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC3072080.10948350466717059No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACGGAGATCGGAAGAGCACA3038170.10827501216591288No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCCGCAGATCGGAAGAGCACACG3022490.10771620466311961No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTAGATCGGAAGAGCACA2983990.10634413266965392No Hit
TGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAGATCGGAAGAGC2974760.10601519177355812No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGGTGAGATCGGAAGAGCACAC2954040.1052767675734384No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)