FastQCFastQC Report
Fri 23 Jun 2023
ERR2098891.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2098891.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73805130
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAG11191371.5163403953085646No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCAGATCGGAAGAGCACACG10536871.4276609227569954No Hit
ACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACT10238281.3872043853862193Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA9108681.2341526937219676No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGAGATCGGAAGAGCAC7081970.9595498307502472No Hit
AGGCTTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA6934030.9395051536390493TruSeq Adapter, Index 4 (100% over 36bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC6542730.886487158819448No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCAGATCGGAAGAGCACAC6216920.8423425309324705No Hit
ATTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA5026740.6810827377446528Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAAC4845450.6565194045454563Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG4482120.60729111919456No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGAGATCGGAAGAGCACACGT3945210.5345441434762055No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCAGATCGGAAGAGCACAC3260420.44176062016285317No Hit
AAGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACT3042770.4122708001462771Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGAGATCGGAAGAGCACACG2944390.398941103416524No Hit
ATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAGATCGGAAGAG2924280.39621636057005793No Hit
CGATGTCGACTTGGAGGTTGTGCAGATCGGAAGAGCACACGTCTGAACTC2536820.34371865478727565Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACAGATCGGAAGAGCACACG2438140.3303483104765211No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGAGATCGGAAGAGCAC2307460.312642224192275No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA2187380.296372352436748No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCAGATCGGAAG2177570.29504317653799944No Hit
ACGATGTCGACTTGGAGGTTGTGCCAGATCGGAAGAGCACACGTCTGAAC2087120.28278793086605225Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAGATCGGAAGAGCAC2011420.27253119126001135No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1947070.26381228513519317Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGT1831960.24821580830492407No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGT1687910.22869819482737855No Hit
AGGCGCTTGAGAGAACTCGGGTGAGATCGGAAGAGCACACGTCTGAACTC1612430.21847126344740536Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGAGATCGGAAGAGCAC1561550.2115774337095538No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCAGATCGGAAGAGCACACGT1458810.19765699213591248No Hit
GGAGCGGCAGTTCAGTCGGTTAGAATACCTGCCAGATCGGAAGAGCACAC1431070.193898445812642No Hit
AGGCTTGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA1425760.19317898362891578TruSeq Adapter, Index 4 (100% over 36bp)
GGAGCGGCAGTTCAGTCGGTTAGAATACCTGCCTGAGATCGGAAGAGCAC1398900.1895396702099163No Hit
AGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA1397460.18934456182110918TruSeq Adapter, Index 4 (100% over 36bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG1396460.1892090698844376No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCAGATCGGAAGAGCACACG1395260.18904647956043164No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT1276710.1729839104680122No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT1254440.1699665050383354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCTTGTAGCTCAGATGGAAGAGCACACGTCTGAACTCCAGTCACTGAC1149910.15580353289805193TruSeq Adapter, Index 4 (100% over 33bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA1130070.15311537287448718Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCAGATCGGAAGA1043470.1413817711587257No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTCGAGATCGGAAGAGCACACGTCT1037610.14058778840983005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCGTAGTTCAATTGGTAGAGCACCGGAGATCGGAAGAGCACACGTC997900.13520740360460037No Hit
AGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCAGATCGGAAGAGCAC976770.13234445898272926No Hit
AGGCTTGTAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA968610.1312388447794889TruSeq Adapter, Index 4 (100% over 36bp)
GCGAAGGTGGCGGAATTGGTAGACGCGCAGATCGGAAGAGCACACGTCTG964100.13062777614509996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA950890.12883792766166796Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGATGTCGACTTGGAGGTTGTGCCCAGATCGGAAGAGCACACGTCTGAA940120.1273786795037147Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGAGAT935960.12681503304716082No Hit
AGAGAACTCGGGTGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC920730.12475149085165219Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCGAAGATCGTCGGTTCAAATCCGGCCCCCGCAGATCGGAAGAGCACA915600.12405641721652681No Hit
TGAGTCTCGTAGAGGGGGGTAGAGATCGGAAGAGCACACGTCTGAACTCC900780.12204842671505355Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAGCGGTAGTTCAGTCGGTTAGAATACCCGCAGATCGGAAGAGCACACG888870.12043471774929466No Hit
AACGATGTCGACTTGGAGGTTGTGCCAGATCGGAAGAGCACACGTCTGAA835560.11321164260533109Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGCGCTTGAGAGAACTCGGGTGAAGGAGATCGGAAGAGCACACGTCTG834520.11307073099119261Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCTCCTAAAGAGTAACGGAGGAGCACGAAGGTTGGCTAATCCTGGTC799780.10836374111122087No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC798210.10815101877064642No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACGGAGATCGGAAGAGCACA785580.10643975561048398No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTAGATCGGAAGAGCACA781170.10584223616976218No Hit
TGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAGATCGGAAGAGC774010.10487211390319345No Hit
GGAGCGGCAGTTCAGTCGGTTAGAATACCTGCAGATCGGAAGAGCACACG758010.10270424291644767No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGGTGAGATCGGAAGAGCACAC757640.10265411089987918No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGAGATCGGAAGAGCACACGT744940.10093336330414973No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCGGAGATCGGAAGAGCAC744130.10082361483544572No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)