FastQCFastQC Report
Wed 27 Sept 2023
ERR2193148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2193148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43451260
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCG7138911.642969616991544No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC6922851.5932449369707575No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC6140201.4131235780044125No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGGAATTCT5327841.226164672785093No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC4892281.1259236210871675No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC4191320.9646026375299589No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTTGGAATTCTCG3683350.8476969367516616No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG3664870.8434438955280008No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA3350390.771068548990294No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG3211890.7391937541051744No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT3115510.717012579151905No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG2885870.6641625582319132No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2795790.6434312836958007No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG2571380.5917849102649727No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG2392320.550575518408442No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC2191300.5043121879549638No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT2114330.4865980871440782No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGT1983930.4565874499381606No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG1891050.43521177521664506No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGT1800170.414296386341846No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC1680090.3866608241049857No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACGTTGGAATTCTCGGGTG1646550.3789418304555495No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGGAATTCTCGG1639410.3772986099827715No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG1635990.37651152118488623No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGTG1603520.3690387804634434No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTG1598950.36798702730369615No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT1569270.36115638533842287No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1553900.35761908860640634No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC1503610.3460452009907192No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTG1488680.34260916714498035No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG1405090.3233715201814631No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC1331360.3064030824422583No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACGTGGAATTCTCGGGTGCCA1330020.3060946909249582No Hit
CGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAA1295560.29816396578603244No Hit
ACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTC1287720.29635964526690367No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA1280120.2946105590493808No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC1255030.288836273102322No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC1253890.2885739101696936No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCTGGAATTCTCGG1228970.28283874851960566No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTCGGGTGC1227390.2824751227006996No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC1207310.2778538527996656No Hit
CATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTTGGAATTCTCGGGTG1151150.26492902622386555No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1024560.23579523355594292No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC1005830.23148465660144266No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC991750.22824424424055825No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCAA950700.21879687723670152No Hit
CGAAACCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGC927010.21334479138234427No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG925520.21300187842654045Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTGGAATT913650.21027008192627786No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC878870.20226571105187743No Hit
CGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCAAG834680.19209569526867576No Hit
CGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTCGGGTGCCA822100.18920049729282878No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA820280.18878163717231675No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTGC806860.18569311914084885No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGGTGGAATT786770.18106954781058135No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAG781600.17987970889681912No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA774170.1781697469762672No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGGAATTCTCGG761090.17515947753874112No Hit
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGC760390.1749983774923903No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCA719970.1656960005302493No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA689700.15872957424019465No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCCCTGGAATTCTCGGGTGC686120.15790566257457206No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGT680330.1565731350483277No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTTGGAATTCTCGGGTGCCA661350.15220502236298786No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGGT660960.15211526662287814No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGGAATTCTCGGG659230.15171711936546836No Hit
GGACACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCA658770.15161125362072353No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG657250.15126143637721898Illumina Small RNA Adapter 2 (100% over 21bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCC655370.15082876768130546No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAA643690.14814069833648094No Hit
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG641710.14768501534823156RNA PCR Primer, Index 1 (100% over 29bp)
GTGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCGGGTGC631130.14525010321910112No Hit
ACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGGAATT618560.14235720667248775No Hit
AACGCGAACTCGGCCTACAATAGTGACGTTGGAATTCTCGGGTGCCAAGG617230.14205111658442127Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT609170.14019616462215365No Hit
AGGACACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCC608990.14015473889594915No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGG608700.14008799744817527No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG606510.13958398444602066No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTG606500.13958168301678708No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGTG606000.13946661155510795No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC603650.13892577568521602No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCGGGTG599730.13802361542565164No Hit
AGGAGCTTGCTCCGTCCACTCCACGCATCGACCTGTGGAATTCTCGGGTG588710.13548744041024355No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTTGGAATTCTCGGGTGC576000.13256232385436004No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTTGGAATTCT575090.13235289379410403No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA570330.13125741347891867No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACGGTGGAATTCTCGGGTGCC569530.13107329914023208No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTTGGAATTCTCGGGTG568190.130764907622932No Hit
CGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATTGGAATTCTCGGGTG560190.12892376423606589No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGGAATTCTC559440.1287511570435472No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTG555010.12773162389307008No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC526110.12108049340801624No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC522460.12024047173775858No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGTGGAATTCTCGGGT522450.12023817030852499No Hit
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGCCA520710.11983772162188161No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC502670.11568594328449855No Hit
CGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGCCAA499500.11495639021745284No Hit
TGATCCTTCGATGTCGGCTCTTCCTATCATTGTGTGGAATTCTCGGGTGC499180.1148827444819782No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC494620.1138332927514645No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACTGGAATTCTCGGGTGCCAA494040.11369980985591673No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA491540.11312445254752107No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC483560.11128791201912211No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC472260.10868729698517374No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGATGGAATTCTCGGGTGCCAAG471820.1085860340988961No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGTGGAATTCTCGG467080.10749515664217793No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGTGGAATTCTCGGGTGCCAA463970.10677941215053371No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGTGGAATTCTCGG456400.10503723022071167No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTTGGAATTCTCGGGT443000.10195331504771092No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGC442140.10175539213362282No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC439890.10123757055606672No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGGTGCCA439840.10122606340989883No Hit
CTCCCGGGGCTACGGCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA439220.1010833747974167No Hit
AGGACACATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGC438710.10096600190650398No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)