FastQCFastQC Report
Wed 27 Sept 2023
ERR2193150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2193150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52071822
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC37653497.231068273355214No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTG21252354.081353250900266No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG18949583.6391236703797305No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC17702183.3995699247858084No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGT13932102.6755545446441262No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG12512802.402988702795919No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT7422831.4254984202396452RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC6958861.336396487144237No Hit
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG6826141.3109086138756583RNA PCR Primer, Index 1 (100% over 29bp)
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCG6589961.265552029272185No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGT5858271.1250364928655656No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGG5780021.1100091715630769No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC5614671.0782549533219714No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTG4679940.8987471189312332No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGGT4418840.8486048366043346No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGGAATTCTCGGG3612070.6936707534451166No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC3324180.6383836540230914No Hit
CCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCCA3240580.6223289056411354RNA PCR Primer, Index 1 (100% over 28bp)
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGT3152510.6054157275311012No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC3130660.60121960011309No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC3101250.5955716318126913No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGGAATTCT2984240.5731007453512957No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA2748530.5278344206968598No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG2745680.5272870997292931No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2230880.4284236491667221No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGGAATTCTCGG2220120.4263572724611019No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGT2043260.39239264568080606No Hit
CCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG2041630.3920796164958469RNA PCR Primer, Index 1 (100% over 29bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGTGGAATTCTCGG1810270.3476486764761179No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGGAATTCTCGGGT1807860.34718585418424575No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGTGGAATTCTCGGGT1788490.34346599202923994No Hit
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGCC1619230.31096088782912185No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC1598470.3069740866758993No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC1472930.2828650781606989No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA1466030.28153998529185326RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCTGGAATTCTCGG1460860.2805471258524428No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1443740.2772593591981475No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1439360.2764182132900977No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGG1433860.27536197984391636No Hit
AGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTG1420850.2728635076375856No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1343060.25792452585968667No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGGAATTCTCG1253960.24081354403154934No Hit
GCGGTTCTATTTTGTTGGTTTTCGGAACTGGAATTCTCGGGTGCCAAGGA1241160.23835540073861827RNA PCR Primer, Index 1 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTGCCAAGG1192000.22891459415420495Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGGAATTCTCGGG1186580.22787372410360443No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT1183030.22719197342470557No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGC1149930.22083536850314167No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGTGGAATTCTCGGGTGCCAAGG1127490.2165259360427219Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1111210.21339948504202522No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTTGGAATTCTCG1107170.21262363356519387No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTG1100390.21132158578971946No Hit
CGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAA1077080.20684507640235825No Hit
CACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAAGGA1073360.2061306785078502RNA PCR Primer, Index 1 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAGG1064100.20435236546937036Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGACTTCTC1019860.1958564077131774No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC997490.19156041822389083No Hit
GGACACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCA920970.17686533035083735No Hit
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGCCA896850.17223326658322038No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA882930.16956003575215786No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC865900.166289552917891No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC865380.16618969084661567No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC865210.16615704363100642No Hit
TCCTCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA836170.16058013103516908No Hit
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGC809310.15542187096890905No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTGC785650.15087814672588182No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC749880.14400878847680804No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG741910.14247821019206894No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC739220.1419616160156639No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG730620.14031005099072585No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA692670.13302204021207478RNA PCR Primer, Index 1 (100% over 23bp)
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCA691580.1328127139472861No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGG677550.13011835844729996Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC673570.12935402951715422No Hit
GACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAAG642060.12330277208275908No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAAGGAA636690.12227150415439661RNA PCR Primer, Index 1 (100% over 23bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTGCCAAG632190.12140731315297551No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGTGGAATTCTCGGGTGCCAAG628570.12071211950294344No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCC604970.1161799178066018No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCA595190.11430174269684668No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAA594460.11416155171217171No Hit
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGCCAA574860.11039751979487103No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC570160.10949492030449788No Hit
AGCGGTCGGCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT568320.10914156220613906RNA PCR Primer, Index 12 (100% over 36bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG555990.1067736788622453Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGGAATTCTC554540.10649521731734296No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG553780.10634926505932517No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCC553690.10633198123929677No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGCATTCTC551220.10585763640073896No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGTGGAATTCTCGGG532180.10220114825250401No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGTGGAATTCTCGGGTGCCAA528820.10155588563810962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)