FastQCFastQC Report
Wed 27 Sept 2023
ERR2193152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2193152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47060340
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC13924932.9589522727630104No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCG8401541.7852697196832832No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG6349401.3492040219003942No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGT4993051.0609889346315815No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTG4939821.049677924128895No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC4897981.0407872106321374No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG4879031.0367604653940026No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC4225470.8978834407061231No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTG3562630.757034479563896No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG3540240.7522767578814773No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGT3223880.6850524241856306No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACGTGGAATTCTCGGGTGCCA3178620.6754349841076371No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGGT3107570.6603373456290371No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAA2908030.6179364619975122No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAG2891180.6143559523794345No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGG2875100.6109390624887112No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCAA2760060.5864938502356761No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGT2611090.5548387453214321No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC2610530.5547197491560835No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGGAATTCTCGG2534550.5385745194361111No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCTGGAATTCTCGG2424300.5151471493831111No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2254170.47899568936391024No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGGAATTCT2234980.47491794576919755No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG2041300.43376227201078443No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGATGGAATTCTCGGGTGCCAAG2004340.4259085250977787No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGGAATTCTCGGG1995240.4239748374108644No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1983360.4214504187602554No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC1854450.39405792648331905No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCA1770430.37620425181798517No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTGC1684540.357953214957648No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAA1680080.3570054954979076No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCA1640120.34851426912767736No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1558050.33107495610953935No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG1538330.3268845911440504No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTG1492160.3170737822973655No Hit
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGC1490730.3167699170894218No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC1488010.3161919357148716No Hit
ACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTC1438300.30562890110866175No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGGAATTCTC1434620.3048469263077997No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACTGGAATTCTCGGGTGCCAA1426320.30308323314281194No Hit
CGAAACCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGC1415670.300820181069665No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC1398860.29724817117768376No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC1360630.2891245579611197No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA1352970.28749686041367317RNA PCR Primer, Index 1 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG1318080.28008297432615237Illumina Small RNA Adapter 2 (100% over 21bp)
AATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAAG1269190.2696941841049172No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGGTGCCA1248210.26523607776739394No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1243800.26429898296527393No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACTGGAATTCTCGGGTGCCAA1243240.2641799867999254No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1223180.2599173741626176No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA1205010.2560563735833613No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC1160650.24663017734253512No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC1158870.2462519395312486No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTG1142880.24285417402424206No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT1104870.23477730930120777No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGC1078220.22911436678953023No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCC1058190.2248581289467947No Hit
CGAGGCCCTGTAATTGGAATGAGTCCACTTTTGGAATTCTCGGGTGCCAA1011810.21500269653810405No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1003440.21322412885244774No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGTG990910.21056158965277344No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTTGGAATTCTCGGGTG975980.20738906688731956No Hit
CATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTTGGAATTCTCGGGTG963200.20467340439954324No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACTGGAATTCTCGGGTG950970.2020746131455914No Hit
AGGAGCTTGCTCCGTCCACTCCACGCATCGACCTGTGGAATTCTCGGGTG927790.19714902187277014No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC912670.19393612540835872No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTTGGAATTCTCGGGTGC891050.1893420234532942No Hit
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGCCA890150.1891507796161269No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGTGGAATTCTCGGGT881180.18724471603902565No Hit
CGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGCCAA880660.1871342195997734No Hit
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG859770.18269523764596685RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT859290.18259324093281093No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCTGGAATTCTCGGGTGCCA839350.17835612747379215No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCCCTGGAATTCTCGGGTGC836730.17779939541448275No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGC834010.17722141403993258No Hit
AGGACACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCC826210.17556396745114888No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGG825360.17538334827160196No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCGGGTGC821810.17462899758055297No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA804300.17090824248188602No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC798990.16977990384259867No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC794020.1687238128751301No Hit
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGCC775990.16489256133721092No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCTGGAATTCTCGGGTGCCAA773330.16432732955180518No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAGG772160.16407871256348766Illumina Small RNA Adapter 2 (100% over 21bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCAAGG722720.15357305110842803Illumina Small RNA Adapter 2 (100% over 21bp)
GGACACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCA722350.15349442864203702No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA717820.15253183466162804RNA PCR Primer, Index 1 (100% over 23bp)
CTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCGGGTGCCAAG694460.14756799462137332No Hit
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGGTG690120.1466457743399219No Hit
CTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTGCC687250.14603591899251045No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC682980.14512857323172762No Hit
CGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAA681250.14476096007806147No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCTGGAATTCTCGGGTGCCA680480.1445973403507072No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG664620.14122719895351374No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCA663510.14099133155434065No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCTGGAATTCTCGGGTGCC660990.1404558488102721No Hit
GAGGGGCTCTCGCTTCTGGCGCCTGGAATTCTCGGGTGCCAAGGAACTCC652230.13859440879517657RNA PCR Primer, Index 1 (100% over 27bp)
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCTGGAATTCTCGGGTGCC651650.13847116276677984No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC626480.13312271012066637No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTTGGAATTCTCGGGT625680.13295271559873983No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA616620.13102752763792186No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTCGGGTGC604840.1285243583025537No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG601770.12787200432466062Illumina Small RNA Adapter 2 (100% over 21bp)
GACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGGTGCCAA599910.12747676706118147No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACGGTGGAATTCTCGGGTGCC597270.1269157851388239No Hit
AGGGGCTCTCGCTTCTGGCGCCTGGAATTCTCGGGTGCCAAGGAACTCCA576880.12258304976122145RNA PCR Primer, Index 1 (100% over 28bp)
TGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCGGGTGCC567020.12048786727847695No Hit
TGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTGGAATTCTCGGGTGCCA554920.11791670013433816No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCC552770.11745983985666061No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC541130.1149864195626296No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGTGGAATTCTCGGGTGCCAAGG538540.11443606229789247Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA532460.11314410393125081No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGGAATTCTCG525150.11159077898714714No Hit
CGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCAAG516670.1097888370547259No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCGGGTG516290.1097080896568108No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC515780.10959971814908265No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGGAATTCTCGGG501980.10666731264584998No Hit
AGGACACATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGC495340.10525635811385978No Hit
TTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCTGGAATTCTCGGG494990.10518198551051693No Hit
AGAGGTGAAATTCTTGGACCGGCGCAAGACGGTGGAATTCTCGGGTGCCA480750.10215608302022466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)