FastQCFastQC Report
Wed 27 Sept 2023
ERR2193154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2193154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36753828
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC30527268.305872248191399No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT18531475.0420516741820744RNA PCR Primer, Index 1 (100% over 30bp)
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG17752584.8301308913999375RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTG14066523.827225833456042No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG13044343.549110585161361No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGT12166543.310278319852833No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG9899832.6935507234783813No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCG8256142.2463347219233873No Hit
CCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCCA6375151.7345540170672833RNA PCR Primer, Index 1 (100% over 28bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC5249651.4283274112291107No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC4635211.261150267123196No Hit
CCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG3972561.0808561219800017RNA PCR Primer, Index 1 (100% over 29bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC3753271.0211915885333087No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTG2899870.7889980874917302No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGT2602700.7081439244913482No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCTGGAATTCTCGG2595250.7061169247459067No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAG2541180.6914055319625482No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2488700.6771267471785524No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC2479560.6746399313834739No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC2446090.665533396956638No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGGT2370510.64496955255926No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC1894710.515513649353749No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGG1861440.506461531027462No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1812140.4930479622421915No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1777040.4834979365958833No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC1679830.4570489909241563No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC1652490.44961030997914014RNA PCR Primer, Index 11 (100% over 50bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1535000.41764357171176836No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGGAATTCTCGG1512650.4115625724754439No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAA1509430.4106864732566088No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGGAATTCTCGGG1493160.4062597234769668No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGTGGAATTCTCGGGT1207000.32840116681179443No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1190990.3240451579628658No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCC1174540.31956943369272994No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1158270.3151426839130879No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAGG1073950.29220085592172873Illumina Small RNA Adapter 2 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGT987690.26873119175504656No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC939200.2555380081769986No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC928610.25265667565294153No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC887280.2414115884745393No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTTGGAATTCTCG872970.23751811648027518No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG872830.23748002520989106No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGGAATTCT859290.2337960552027397No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGTGGAATTCTCGG841800.22903736720975024No Hit
CACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAAGGA841420.22893397661870757RNA PCR Primer, Index 1 (100% over 22bp)
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGC808270.21991450795274983No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGC806140.2193349764819055No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGG761080.20707502902826883Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA746100.20299926309716637No Hit
TAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGTGGAATTCTCGGGTG743040.20216669675877028No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAA722910.19668971623853712No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGGAATTCTCG714390.19437159035515975No Hit
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGC713190.19404509375186715No Hit
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGCCA686460.18677238191352477No Hit
CATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTTGGAATTCTCGGGTG684840.18633161149907979No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG672000.18283809784384908Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC665850.18116480275197458No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGT655820.178435835309454No Hit
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGCC596210.16221711654089474No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC586900.15968404706034972No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTTGGAATTCTCGGGT560360.15246303051752869No Hit
AGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTG558060.1518372453612179No Hit
CGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAA548190.14915181079913636No Hit
CCCCGCGAGGGGGGCCCGGGCACTGGAATTCTCGGGTGCCAAGGAACTCC546690.1487436900450206RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC519940.14146553659662336No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAAG511090.13905762414734052No Hit
AGGGGCTCTCGCTTCTGGCGCCTGGAATTCTCGGGTGCCAAGGAACTCCA510040.1387719396194595RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC500030.13604841378699384No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT492710.13405678450690905No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA485020.13196448544080905No Hit
GCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGT483650.13159173515205003No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCAAGG479640.13050069233604727Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGGAATTCTC478260.1301252212422608No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTGCCAAGG477820.13000550582105352Illumina Small RNA Adapter 2 (100% over 21bp)
ATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAGGA464840.12647390089543872RNA PCR Primer, Index 1 (100% over 22bp)
ACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAAGGAA462940.12595694794022544RNA PCR Primer, Index 1 (100% over 23bp)
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGGAATTCTCGGGT461890.12567126341234441No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTGGAATTCTCGGGTGCCAAG459990.12515431045713116No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCAA453450.12337490396918656No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC446250.12141592434943102No Hit
CCCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTG444410.1209152962243824No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGTGGAATTCTCGGGTGCCAAGG438110.11920118905709631Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAA437250.11896719982473662No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGGAATTCTCGGG435490.1184883381399075No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC431310.11735104163843832No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGG430260.1170653571105573No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA418510.11386841120331738No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGTGGAATTCTCGGGTGCCAA418260.11380039107763143No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCC411330.11191487319361672No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT408390.11111495651554988No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG405920.11044291767377265No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTG400300.10891382524835236No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCA397580.10817376628088916No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGG394720.10739561604304183No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTG386670.10520536799595405No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGTGGAATTCTCGGG386100.10505028210939008No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCA378200.10290084613771386No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT377950.10283282601202792No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAG369690.10058544105936394No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)