FastQCFastQC Report
Wed 27 Sept 2023
ERR2193156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2193156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58732724
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA18241333.1058205303060693RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC7783661.3252680056181287No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG6859861.1679792001474338No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCAA5872600.9998855152708396No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA5833750.9932708041942682RNA PCR Primer, Index 1 (100% over 23bp)
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCG5752080.9793654385926319No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACGTGGAATTCTCGGGTGCCA5302760.9028629423011266No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC5044600.8589078892373526No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA4195750.7143802831280225No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCA4138310.7046003859790327No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG3969330.6758293723955321No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC3646370.6208412877291372No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAA3617140.6158645051096217No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG3509540.5975442242386033No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGGAATTCTCGG3493030.5947331848596022No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC3192990.5436475243341344No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCTGGAATTCTCGG3056230.5203623792419367No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAG2949460.5021834165226187No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGGAATTCTCGGG2936540.49998362071542946No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGATGGAATTCTCGGGTGCCAAG2835850.48283985602302393No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC2824990.4809908016525847No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACTGGAATTCTCGGGTGCCAA2776450.4727262437206216No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA2444230.4161615252171856No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC2434360.41448103105178635No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGT2381700.40551499024632337No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGGTGCCA2206040.37560662093588576No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2071800.35275053818379No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG2036870.346803257414044Illumina Small RNA Adapter 2 (100% over 21bp)
AGGAGCTTGCTCCGTCCACTCCACGCATCGACCTGTGGAATTCTCGGGTG1872930.3188903685107471No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC1766010.3006858663664229No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCA1647240.2804637496466195No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA1635530.2784699718678125No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC1598420.2721515181213117No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATTGGAATTCTCGGGTGCCA1506900.2565690636109437No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACGGTGGAATTCTCGGGTGCC1412580.24050987316712913No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGA1396590.23778737046148243RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTG1396300.23773799423980405No Hit
AGAGGTGAAATTCTTGGACCGGCGCAAGACGGTGGAATTCTCGGGTGCCA1367540.23284123515197425No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC1345820.2291431264110958No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAAG1277800.2175618484850115No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAA1274160.21694209177153095No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1263610.21514581887943762Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGGGCCGCCGGTGAAATACCACTACTCTGATTGGAATTCTCGGGTGCC1244930.21196530915201547No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGTGGAATTCTCGGGTGCCA1232050.20977232385816125No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTGGAATTCTCGGGTGCCAAGG1221850.20803564295774876Illumina Small RNA Adapter 2 (100% over 21bp)
GTGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCGGGTGC1217700.20732905219924755No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTG1211420.20625980160566024No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACTGGAATTCTCGGGTGCCAA1208290.20572687893720032No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1187690.20221946456970052No Hit
CGAAACCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGC1161850.19781987295532216No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTG1161140.19769898634362676No Hit
CATGACCCGCCGGGCAGCTTCCGGGAAACCTGGAATTCTCGGGTGCCAAG1152170.196171728728264No Hit
CACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGTGGAATTCTCGGGTGCC1151380.1960372210898987No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC1119790.19065861818362112No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATCTGGAATTCTCGGGTGCC1108880.1888010506715132No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1104080.18798378907131907No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCC1098360.1870098856644211No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC1083310.18444743002214575No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG1058530.18022831701114356No Hit
ATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCAAG1030600.175472876075014No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTG1021810.1739762657696585No Hit
CATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTTGGAATTCTCGGGTG1021320.17389283698130534No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGC1005970.17127930248901788No Hit
TGCGGGCCGCCGGTGAAATACCACTACTCTGATCTGGAATTCTCGGGTGC1005550.1712077920990009No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA994540.16933319830355562No Hit
ATGGTTCCTTTGGTCGCTCGCTCCTCTCCTTGGAATTCTCGGGTGCCAAG985150.16773443029817586No Hit
ATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAGGA982970.16736325732142102RNA PCR Primer, Index 1 (100% over 22bp)
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGT976950.1663382750645109No Hit
ATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAAGG971500.1654103426226238Illumina Small RNA Adapter 2 (100% over 21bp)
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTTGGAATTCTCGGGTGC965450.16438025248071247No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGG965370.16436663145404257Illumina Small RNA Adapter 2 (100% over 21bp)
GACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGGTGCCAA964080.1641469923989904No Hit
CCTACTTGGATAACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAG956790.16290577634369555No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCA948890.16156069996004274No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGTGGAATTCTCGGGTGCCAAGG920450.15671842497889252Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGT914670.1557343058019921No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG905380.1541525640799497Illumina Small RNA Adapter 2 (100% over 21bp)
TAGAGGTGAAATTCTTGGACCGGCGCTGGAATTCTCGGGTGCCAAGGAAC902740.15370307019984294RNA PCR Primer, Index 1 (100% over 24bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCC898700.15301520835301288No Hit
CGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGTGGAATTCTCGGG894610.15231883336451416No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCCCTGGAATTCTCGGGTGC879800.14979724080224852No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGGTGCC858710.14620639764639556No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAGG853740.14536019136452788Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTG842460.1434396266040717No Hit
CGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGCCAA836880.14248955999384602No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTGC828860.14112405207018833No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG821880.13993561749323938No Hit
GAGCGGTCGGCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC818620.13938056065644086RNA PCR Primer, Index 6 (100% over 35bp)
GGCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAA789740.1344633700286062RNA PCR Primer, Index 1 (100% over 23bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAAC767640.13070056141104575RNA PCR Primer, Index 1 (100% over 24bp)
CATGACCCGCCGGGCAGCTTCCGGGAAACCAAATGGAATTCTCGGGTGCC764290.13013018091924358No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCA759690.12934697188572422No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACGGATGGAATTCTCGGGTGC701270.11940021716002819No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTCGGGTGCCAAGG698850.11898818110326365Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA683620.11639507815098105No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCAAGGAA681160.11597623158088155RNA PCR Primer, Index 1 (100% over 23bp)
CCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCTGGAATTCTCGGGT675610.11503127285565709No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCGGGTGCC674220.11479460751726756No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAG666740.11352104152363168No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTGGAATTCTCGGGTGCCAAG658870.11218107302498007No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCTGGAATTCTCGGGTGCCA652660.11112374082972891No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA642300.1093598178759766No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCTGGAATTCTCGGGTGCC633840.10791939430563446No Hit
CGAGGCCCTGTAATTGGAATGAGTCCACTTTTGGAATTCTCGGGTGCCAA632990.10777467089726674No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAA631170.10746479254052647No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGTGGAATTCTCGGGTGCCAAGG630260.10730985336215634Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGCGGCGACGACCCATTCGAACGTCTGTGGAATTCTCGGGTGCCAAG629150.10712086161711144No Hit
ATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCAAGGAACTCC625190.10644662079695129RNA PCR Primer, Index 1 (100% over 27bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC616530.10497214465993439No Hit
CGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGGAATTCTCGGGTGCCA612020.10420425928141866No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTGGAATTCTCGGGTGCCA611040.10403740170471235No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTTGGAATTCTCGGGTGCCAAGGAA610090.10387565201300725RNA PCR Primer, Index 1 (100% over 23bp)
CGGGCCGATCGCACGCCCCCCGTGGCGGCGACGTGGAATTCTCGGGTGCC609410.10375987328631309No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGT608920.10367644449795996No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC606530.10326951632619662No Hit
CGAGGCCCTGTAATTGGAATGAGTCCACTTGGAATTCTCGGGTGCCAAGG600460.1022360209276178Illumina Small RNA Adapter 2 (100% over 21bp)
AATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTTGGAATTCTCGGGTGCC599980.10215429476759838No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGC596980.10164350626747705No Hit
CACCCAGGCGGCCCGGGTTCGACTCCCGGTATGTGGAATTCTCGGGTGCC596090.1014919723457744No Hit
CCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGTGGAATTCTCGGG595040.10131319637073193No Hit
CATGACCCGCCGGGCAGCTTCCGGGAAACCATGGAATTCTCGGGTGCCAA592890.10094713127897831No Hit
AGAGGTGAAATTCTTGGACCGGCGCAAGACGTGGAATTCTCGGGTGCCAA590000.10045507169052809No Hit
TTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTATGGAATTCTCGGGTGC588810.1002524589188133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)