FastQCFastQC Report
Wed 27 Sept 2023
ERR2193158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2193158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38200240
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC10792642.8252806788648446No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCG5886781.541032202939039No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGT4521361.1835946580440333No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG4414971.1557440476813758No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTG3549210.9291067281252683No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTG2822160.7387806987600077No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGGAATTCTCGG2557780.6695717094971131No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGGAATTCTCGGG2444540.639927916683246No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACGTGGAATTCTCGGGTGCCA2419540.6333834551824805No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC2253440.589902052971395No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2252970.5897790170951805No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTG2216220.5801586586890554No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC2186520.572383838426146No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG2077060.5437295681911947No Hit
CGAAACCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGC2020720.5289809697530696No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGGT1935560.5066879160968621No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCTGGAATTCTCGG1894210.4958633767745962No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1891270.49509374810210616No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGT1881610.4925649681782104No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGG1860350.4869995581179594No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACTGGAATTCTCGGGTGCCAA1816190.47543942132300737No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTGC1742590.4561725266647539No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGT1736570.45459662033536963No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAA1709350.44747101065333617No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAG1697330.44432443356376816No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC1586970.4154345627147892No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGGAATTCT1568090.4104921853894112No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTG1562790.40910475955124886No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCAA1519460.39776189887812224No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC1474300.3859399836231395No Hit
CTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCGGGTGCCAAG1405900.3680343369570453No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG1374780.3598877912808925No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1359520.35589305198082527No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACTGGAATTCTCGGGTG1341030.35105276825485915No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCCA1199320.3139561426839203No Hit
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGC1178190.3084247638234734No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTTGGAATTCTCG1121780.2936578408931462No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCTGGAATTCTCGGGTGCCA1066040.2790663095310396No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAA1041750.27270771073689587No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCTGGAATTCTCGGGTGCC1016670.266142306959328No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG996200.2607837018825013No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCTGGAATTCTCGGGTGCCA992430.25979679708818587No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGTGGAATTCTCGGGT990730.25935177370613377No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGGAATTCTC974480.25509787373063625No Hit
CATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTTGGAATTCTCGGGTG950340.24877854170549715No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCA940650.24624190842780047No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA923990.24188067928369036No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCTGGAATTCTCGGGTGCC908150.2377341084768054No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGATGGAATTCTCGGGTGCCAAG897220.23487286990867073No Hit
GAGGGGCTCTCGCTTCTGGCGCCTGGAATTCTCGGGTGCCAAGGAACTCC893040.23377863594574275RNA PCR Primer, Index 1 (100% over 27bp)
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCTGGAATTCTCGGGTGCCAA883710.23133624291365706No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCC866060.22671585309411668No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGG847690.22190698278335425No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC845350.22129442118688258No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA835470.21870805000178012No Hit
CTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTGCC828360.2168468051509624No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGGTGCCA798760.2090981627340561No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCTGGAATTCTCGGGTGCC795780.2083180629231649No Hit
TCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTTGGAATTCTCGGGTG792520.2074646651434651No Hit
GAGCGGTCGGCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC787560.2061662439817132RNA PCR Primer, Index 5 (100% over 35bp)
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTTGGAATTCTCGGGTGC748840.1960301820093277No Hit
CGAGGCCCTGTAATTGGAATGAGTCCACTTTTGGAATTCTCGGGTGCCAA748830.19602756422472736No Hit
CCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGCCA747680.19572651899569218No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCTGGAATTCTCGGGTGC744080.19478411653958194No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA705210.18460878779819184No Hit
CTTTCCCGCCCCCCGTTCCTCCCGACCCCTGGAATTCTCGGGTGCCAAGG703200.1840826130935303Illumina Small RNA Adapter 2 (100% over 21bp)
GTGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCGGGTGC691810.18110095643378157No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG687940.18008787379346308No Hit
CTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTTGGAATTCTCGGGT682540.17867427010929776No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCC682470.17865594561709558No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA672340.17600412981698543No Hit
AGGAGCTTGCTCCGTCCACTCCACGCATCGACCTGTGGAATTCTCGGGTG633960.16595707252101033No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACTGGAATTCTCGGGTGCCAA627930.16437854840702573No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGC620160.16234452977258781No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC618350.1618707107599324No Hit
AGGGGCTCTCGCTTCTGGCGCCTGGAATTCTCGGGTGCCAAGGAACTCCA608200.15921365939062163RNA PCR Primer, Index 1 (100% over 28bp)
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG605940.15862204007095243RNA PCR Primer, Index 1 (100% over 29bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC590170.1544937937562696No Hit
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGATGGAATTCTCGGGTG588490.15405400594341814No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC583000.15261684219785007No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCGGGTG579690.15175035549514873No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGCCAAG571340.14956450535389304No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGGAATTCTCGG555570.14543625903921023No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT534740.13998341371677248No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCCCTGGAATTCTCGGGTGC526320.13777923908331466No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC519960.13611432807751994No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA516590.13523213466721673No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTTGGAATTCTCGGGTGC509280.13331853412439293No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC496660.13001488995880653No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG490850.12849395710602865No Hit
CCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCCA484090.1267243347162217RNA PCR Primer, Index 1 (100% over 28bp)
CTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTGCCAAGGAACT483150.1264782629637929RNA PCR Primer, Index 1 (100% over 25bp)
GACCGCGGTTCTATTTTGTTGGTTTTCGGAACTTGGAATTCTCGGGTGCC476660.1247793207581942No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGACGGTGGAATTCTCGGGTGCC476630.12477146740439327No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT471370.12339451270463223No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGGAATTCTCGGG466720.12217724286548985No Hit
GAAACCCCCCCCGAGTGTTACAGCCCCCCCGGCTGGAATTCTCGGGTGCC454830.1190646969757258No Hit
CTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTGGAATTCTCGGGTGCCAA440400.11528723379748401No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGTG440060.11519822912107358No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCTGGAATTCTCGGG439680.11509875330626194No Hit
CTTTCCCGCCCCCCGTTCCTCCCGACCCTGGAATTCTCGGGTGCCAAGGA437050.11441027595638144RNA PCR Primer, Index 1 (100% over 22bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC436070.11415373306555142No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC432290.11316421048663569No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCGGGTGCC424980.11125060994381186No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC424410.11110139622159441No Hit
TAGAGGTGAAATTCTTGGACCGGCGCAAGTGGAATTCTCGGGTGCCAAGG423400.1108369999769635Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGGTGAAATTCTTGGACCGGCGCAAGACGTGGAATTCTCGGGTGCCAA423160.11077417314655615No Hit
CGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGCCAA415470.10876109678892071No Hit
GACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGGTGCCAA411600.10774801414860222No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA410690.10750979574997435No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGGAATTCTCGGGT410550.10747314676557006No Hit
AGAGGTGAAATTCTTGGACCGGCGCAAGACGGTGGAATTCTCGGGTGCCA406280.1063553527412393No Hit
CTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTTGGAATTCTCGGGTGC402780.10543912813113217No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTGGAATTCTCGGGTGCC397460.10404646672376927No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT396730.10385536844794692No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC395970.10365641681832365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)