FastQCFastQC Report
Thu 22 Jun 2023
ERR2213253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2213253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25778593
Total Bases968.4 Mbp
Sequences flagged as poor quality0
Sequence length1-76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG422302316.381898732797403No Hit
AACGATGCCGACTGGCGATGCGGCGGCG2059950.7990932631583113No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1833410.711214145783674No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1561900.605890321477204No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1347750.5228175176201432No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1286580.4990885266701717No Hit
AAACGATGCCGACTGGCGATGCGGCGGCG1258140.48805611694943934No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA993100.3852421270625592No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTC909850.35294788974712465No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT752260.29181577132623177No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAA711770.2761089404685508No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC656310.2545949656755898No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC618810.24004801192989855No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT605860.2350244639030532No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGT579240.22469806633744516No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT579150.22466315364845554No Hit
AACGATTAAAGTCCTACGTGATCTGAGTT576810.2237554237347244No Hit
AGCTAGGAATAA568720.2206171609133206No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGT546360.2119432972932231No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC537760.20860719590087792No Hit
GCCGCCTGGATACCGCAGCTAGGAATAA502840.19506107257289024No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT496450.19258227165462444No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCC469330.18206191470574054No Hit
CTGAGCGTCGCT462910.1795714762244782No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGC462320.17934260415221265No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCG430400.16696023712388025No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC414940.16096301299298996No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT412760.1601173500819071No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTT411740.15972167294002432No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG389030.1509120377516337No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT387420.15028748853748536No Hit
ATGTACACGGAGTCGACCCGCAACGCGA373780.14499627656171923No Hit
GCTGAGAAGACGGTCGAACTTGACTATCT369990.14352606443648805No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG368050.14277350202937764No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC346280.13432851048154568No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTG335260.1300536456741452No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCC333550.1293903045833417No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG332000.12878903049518645No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330740.12830025284933125No Hit
TAAACTTTAAGGATGTCTTTTGTTCCTGGG327810.12716365086333456No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACA309390.12001818718345102No Hit
AGCTAGGAATAATGGAAT309330.11999491205745791No Hit
TAAACTTTAAGGATGTCTTTTGTTCCTGG305810.11862943799919567No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC305310.11843547861591981No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCC277120.10750004858682552No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAG276140.10711988819560479No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCC275130.1067280902413875No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAA274660.10654576842110816No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC268300.10407860506583894No Hit
TCTGAGCGTCGCT264500.10260451375294223No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTT261900.10159592495990763No Hit

[OK]Adapter Content

Adapter graph