FastQCFastQC Report
Thu 22 Jun 2023
ERR2382483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394190
Total Bases53.6 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10497426.63030518277988Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA152973.880615946624724Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA146343.7124229432507163Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA100252.5431898323143662Illumina PCR Primer Index 6 (100% over 28bp)
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC57971.4706106192445267No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC55141.3988178289657272No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC52211.3244881909738957No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41131.043405464369974Illumina PCR Primer Index 6 (96% over 29bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC40081.0167685633831403No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG39881.0116948679570765No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC38030.9647631852659886No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC36330.9216367741444482No Hit
CTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA32030.8125523224840814Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA31210.7917501712372207Illumina PCR Primer Index 6 (96% over 29bp)
GCTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA29920.7590248357391105Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT27300.6925594256576777No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA25920.6575509272178391No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC25700.6519698622491692No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA25060.6357340368857658Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC24510.6217813744640909No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA22610.573581267916487Illumina PCR Primer Index 6 (96% over 29bp)
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG22070.5598822902661154No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC21710.5507496384992009No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA20370.5167558791445749Illumina PCR Primer Index 6 (96% over 29bp)
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC20320.5154874552880591No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC19440.49316319541337933TruSeq Adapter, Index 6 (96% over 26bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA19120.4850452827316777Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC18760.4759126309647632No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC18720.4748978918795505No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA18660.47337578325173135No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC18420.4672873487404551No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC17710.4492757299779294No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA17360.4403967629823181No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCACTGTAGGCACCA17130.43456201324234506No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC16150.4097009056546336No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG16020.4064030036276922No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG15990.40564194931378267No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACCA14600.37037976610264084No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC13340.3384154849184403No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA12840.32573124635328143Illumina PCR Primer Index 6 (96% over 29bp)
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG12030.3051827798777239No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC11320.28717116111519825No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC10490.2661153250970344No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA10390.26357847738400264No Hit
GGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCTT10260.26028057535706134No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA9930.2519089779040564Illumina PCR Primer Index 6 (100% over 28bp)
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC9320.2364342068545625No Hit
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG8250.20928993632512238No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC7970.2021867627286334No Hit
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA7870.19964991501560161Illumina PCR Primer Index 6 (96% over 29bp)
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAG7140.18113092671046957No Hit
GCCCCCTATGAGGAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT7120.1806235571678632TruSeq Adapter, Index 6 (95% over 22bp)
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC6950.17631091605570917No Hit
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC6940.17605723128440598No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC6890.1747888074278901No Hit
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT6750.1712372206296456RNA PCR Primer, Index 6 (95% over 22bp)
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT6550.16616352520358202No Hit
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCA6510.16514878611836933No Hit
TGGTAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC6500.16489510134706614No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG6370.1615971993201248No Hit
TCCTTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG6350.16108982977751846No Hit
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG6240.1582992972931835Illumina PCR Primer Index 6 (96% over 29bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA6170.15652350389406122No Hit
ACCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC5950.1509424389253913No Hit
TGGTAATGTGGAGATCATCGGTTCATCCGATTGGAAGCACCACTGTAGGC5950.1509424389253913No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC5700.14460031964281186No Hit
GCCCCCTACAGGGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC5690.14434663487150864No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5560.14104873284456734Illumina PCR Primer Index 6 (96% over 29bp)
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG5370.13622872218980694No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5360.13597503741850375Illumina PCR Primer Index 6 (100% over 28bp)
TCCCGCTAGAGACCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT5130.13014028767853067Illumina PCR Primer Index 6 (95% over 21bp)
TATTACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA5090.12912554859331793No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC5070.1286181790507116No Hit
TCCTGGGCGAAATCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC5060.1283644942794084No Hit
TGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCCCACTGTAGGCACC5010.12709607042289253No Hit
TCTTACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT5000.12684238565158934No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCAACCCTCACTGGGGTCGCCA5000.12684238565158934No Hit
CAATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA4950.12557396179507346Illumina PCR Primer Index 6 (96% over 29bp)
ACCCCGCGGGTATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4910.12455922270986072No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCCA4880.12379816839595119No Hit
TCCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4860.12329079885334483No Hit
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4830.12252974453943528No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA4820.12227605976813212Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA4770.12100763591161622Illumina PCR Primer Index 6 (96% over 29bp)
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGAGCCCCCTATGAGGAGCCA4740.1202465815977067No Hit
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT4450.1128897232299145Illumina PCR Primer Index 6 (95% over 21bp)
CCCCCCATCGTGAGTGCCACTGTAGGCACCATCAATATCTCGTATGCCGT4300.10908445166036684No Hit
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG4230.10730865826124457No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4210.10680128871863823Illumina PCR Primer Index 6 (96% over 29bp)
CCTTATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC4080.10350338669169691No Hit
GGCCATCTTAGTATAGTGGTTAGTACACATCGTTGTGGCCGATGAAACCC4060.10299601714909054No Hit
TTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCC3990.1012202237499683No Hit
TGGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCT3980.10096653897866512No Hit
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3960.10045916943605875Illumina PCR Primer Index 6 (96% over 29bp)
GCCCAGTTGGTTAAGGCACCGTGCTGATAACGCGGGGATCAGCGGTTCGA3950.10020548466475557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)