FastQCFastQC Report
Thu 22 Jun 2023
ERR2382484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA56737114.184275Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA52556213.13905Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA45288511.322125Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2654036.635075Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA2212205.5305Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC773791.9344750000000002No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA662871.657175Illumina PCR Primer Index 6 (100% over 28bp)
CAATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA479191.197975Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT390620.97655No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC384700.9617499999999999TruSeq Adapter, Index 6 (96% over 26bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT369240.9230999999999999No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC335870.839675No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA240350.600875Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG230920.5773No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA224880.5622Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA224710.561775Illumina PCR Primer Index 6 (96% over 29bp)
GCTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA220240.5506Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA204980.51245Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA202550.506375No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA196320.4908Illumina PCR Primer Index 6 (96% over 29bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC151880.3797No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC145360.3634No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA144930.362325Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA136490.341225Illumina PCR Primer Index 6 (96% over 28bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA121400.3035Illumina PCR Primer Index 6 (96% over 29bp)
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG99870.24967499999999998No Hit
CTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA94010.23502499999999998Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC92810.23202499999999998No Hit
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT92690.231725No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA84870.212175Illumina PCR Primer Index 6 (100% over 28bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA84350.210875Illumina PCR Primer Index 6 (100% over 28bp)
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC84010.21002500000000002No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA80040.20010000000000003Illumina PCR Primer Index 6 (96% over 29bp)
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC79110.19777499999999998No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC77950.194875No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA74250.185625Illumina PCR Primer Index 6 (96% over 29bp)
ATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT69770.174425No Hit
GCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG69480.1737No Hit
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG66260.16565Illumina PCR Primer Index 6 (96% over 29bp)
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG62090.155225No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA60250.150625No Hit
CCTTCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC59380.14845No Hit
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA59090.147725Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC56580.14145No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC56500.14125No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT55490.13872500000000001No Hit
AGATCGGAAGAGCGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT51270.12817499999999998Illumina Single End PCR Primer 1 (95% over 23bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA49850.124625No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC49050.12262499999999998No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA48180.12045Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA47320.1183Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA46900.11725TruSeq Adapter, Index 6 (96% over 29bp)
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC45180.11295000000000001No Hit
TCCTGCGGAAGGATCATTCTGTAGGCACCATCAATATCTCGTATGCCGTC44720.11180000000000001No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA41890.104725Illumina PCR Primer Index 6 (96% over 29bp)
GCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA40850.10212500000000001Illumina PCR Primer Index 6 (96% over 29bp)
AGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA40800.10200000000000001Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)