FastQCFastQC Report
Thu 22 Jun 2023
ERR2382485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA55186313.796575Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA44909411.22735Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA43098510.774625Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA2216835.542075Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2165295.413225Illumina PCR Primer Index 6 (100% over 28bp)
CAATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1268563.1713999999999998Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA659981.64995Illumina PCR Primer Index 6 (100% over 28bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC632781.58195No Hit
GCTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA586571.466425Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT403741.00935No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT380280.9507No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC337250.8431249999999999No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC318570.796425TruSeq Adapter, Index 6 (96% over 26bp)
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG231760.5793999999999999No Hit
CTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA214550.5363749999999999Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA212790.531975Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA202560.5064Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA200210.500525Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA189530.47382500000000005Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA187230.468075Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA176010.44002499999999994No Hit
CCTTCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC173960.43489999999999995No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC147450.368625No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA142000.35500000000000004Illumina PCR Primer Index 6 (96% over 29bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC128390.320975No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA123650.309125Illumina PCR Primer Index 6 (96% over 29bp)
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG100620.25155No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA99590.24897500000000003Illumina PCR Primer Index 6 (96% over 28bp)
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT96390.240975No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC93990.234975No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC85700.21425No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC79130.197825No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC78220.19555000000000003No Hit
GCGTCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC78050.19512500000000002TruSeq Adapter, Index 6 (96% over 26bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA76080.1902Illumina PCR Primer Index 6 (96% over 29bp)
AGCGGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA75160.1879Illumina PCR Primer Index 6 (96% over 29bp)
GCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG70980.17745No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA68720.17179999999999998Illumina PCR Primer Index 6 (100% over 28bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA67800.16949999999999998Illumina PCR Primer Index 6 (100% over 28bp)
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG66180.16544999999999999Illumina PCR Primer Index 6 (96% over 29bp)
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG64580.16145No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA64180.16045Illumina PCR Primer Index 6 (96% over 29bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA59330.14832499999999998No Hit
CAATCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA59160.1479Illumina PCR Primer Index 6 (96% over 29bp)
ATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT58080.1452No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC57990.14497500000000002No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC56480.1412No Hit
AGATCGGAAGAGCGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT50360.12589999999999998Illumina Single End PCR Primer 1 (95% over 23bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA49890.124725Illumina PCR Primer Index 6 (96% over 29bp)
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC48320.1208No Hit
GTATGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA46820.11705000000000002Illumina PCR Primer Index 6 (96% over 29bp)
CAACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA46820.11705000000000002Illumina PCR Primer Index 6 (96% over 29bp)
TATTACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA46530.116325No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT46090.11522500000000001No Hit
TCCTGCGGAAGGATCATTCTGTAGGCACCATCAATATCTCGTATGCCGTC45910.11477499999999999No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC45190.11297499999999999No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA43960.1099No Hit
AGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA41600.104Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA40680.10169999999999998Illumina PCR Primer Index 6 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)