FastQCFastQC Report
Thu 22 Jun 2023
ERR2382486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396867
Total Bases461.9 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA48086414.156103256324137Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA42033712.3742554536283Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA38002611.18754428713282Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2109026.2087211539339044Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA1866115.493621033734909Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC630071.854856254307278No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA553951.6307674100870009Illumina PCR Primer Index 6 (100% over 28bp)
CAATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA422701.2443819554901618Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT336630.9910014139499721No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT318450.9374815086961015No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC312670.9204658292479512TruSeq Adapter, Index 6 (96% over 26bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC291350.857702111975535No Hit
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG200450.5901025857061817No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA192910.5679056613049613Illumina PCR Primer Index 6 (96% over 29bp)
GCTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA184570.5433536255614365Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA176300.5190076620603633Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA175050.5153278005880124Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA170440.5017564714779825No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA167810.4940140429401563Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA158630.4669891402872117Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAA137280.40413710633945926RNA PCR Primer, Index 37 (96% over 27bp)
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC125490.3694286529322461No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC124310.3659548637023469No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA117150.34487661718872126Illumina PCR Primer Index 6 (96% over 29bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA102010.30030613503560777Illumina PCR Primer Index 6 (96% over 29bp)
CTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA86730.2553235083975911Illumina PCR Primer Index 6 (96% over 29bp)
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG85140.2506427246047608No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA82230.242076007097128Illumina PCR Primer Index 6 (96% over 28bp)
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT81340.23945594572881423No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC81060.23863165675900763No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC72290.2128137486689941No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC68600.20195079760261442No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA67500.19871251950694568Illumina PCR Primer Index 6 (100% over 28bp)
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC66020.19435556352368225No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA65610.1931485689607512Illumina PCR Primer Index 6 (100% over 28bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA65230.19202989107315652Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA62900.18517062928869454Illumina PCR Primer Index 6 (96% over 29bp)
GCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG60320.1775753952097624No Hit
CCTTCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC59990.1766039117810618No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT58840.17321843922649902No Hit
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG55600.1636802382901656Illumina PCR Primer Index 6 (96% over 29bp)
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG52640.15496632632363883No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA51910.1528172872237859No Hit
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA48220.1419543361574062Illumina PCR Primer Index 6 (96% over 29bp)
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC47530.13992305262466856No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC47320.13930483589731363No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT45840.13494787991405022No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCGTCTGCTTGAAAAA45070.13268108524708208TruSeq Adapter, Index 6 (96% over 28bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA42920.12635172351463864No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC42360.12470314557502546No Hit
AGATCGGAAGAGCGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT41970.123555028795652Illumina Single End PCR Primer 1 (95% over 23bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA38610.11366356115797291Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTCTGTAGGCACCATCAATATCTCGTATGCCGTC38560.11351636669907889No Hit
CAAGCTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA38450.11319253888951201TruSeq Adapter, Index 6 (96% over 29bp)
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC38290.11272151662105111No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA37430.11018977192807373Illumina PCR Primer Index 6 (100% over 28bp)
AGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA35400.10421367689697596Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)