FastQCFastQC Report
Fri 23 Jun 2023
ERR2382493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA112804328.201074999999996Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3728099.320225Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA2557366.393400000000001Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1436263.59065Illumina PCR Primer Index 6 (100% over 28bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG796641.9916No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA731031.827575Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC713131.782825No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA644961.6124Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCT520551.301375No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCG438011.095025No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA413201.0330000000000001Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA396250.990625Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA393100.9827499999999999Illumina PCR Primer Index 6 (100% over 28bp)
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTC287160.7179No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGT281530.703825No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA233110.5827749999999999Illumina PCR Primer Index 6 (96% over 29bp)
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT226560.5664No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA220920.5523Illumina PCR Primer Index 6 (96% over 29bp)
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA211930.529825No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA208120.5203Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATATCTCGT204470.511175No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA191890.479725No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA158650.396625Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA153280.3832Illumina PCR Primer Index 6 (100% over 28bp)
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT106740.26685No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTAT94220.23554999999999998No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCCCCATCAATATCTC86910.21727500000000002No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATATCT82840.2071No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTCGGCACCATCAATATCTC75810.189525No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATG70560.1764No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCCCCATCAATATCT67340.16835No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA65930.164825No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTAT64630.161575No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCCTCAATATCTCG63760.1594No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTC63750.159375No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTCGGCACCATCAATATCTCG60740.15185No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCT59450.148625No Hit
CCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT59270.148175No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA58230.145575Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA58180.14545Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG58110.14527500000000002Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA55770.13942500000000002Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT50220.12555000000000002No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA48230.120575Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCT44140.11035No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA43540.10885No Hit
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA43540.10885Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA42550.106375Illumina PCR Primer Index 6 (100% over 28bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA42510.106275Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)