FastQCFastQC Report
Thu 22 Jun 2023
ERR2382494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA112133928.033475000000003Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3573368.933399999999999Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA2506586.26645Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1405773.514425Illumina PCR Primer Index 6 (100% over 28bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG745421.86355No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA711881.7797Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC676561.6913999999999998No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA640631.601575Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCT506411.266025No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCG407841.0196No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA392200.9804999999999999Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA375960.9399Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA375350.938375Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTC270380.6759499999999999No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGT269760.6744No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA221430.553575Illumina PCR Primer Index 6 (96% over 29bp)
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT221270.553175No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA211070.527675Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATATCTCGT201280.5032No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA199410.498525No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA191130.47782500000000006Illumina PCR Primer Index 6 (96% over 29bp)
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA183250.458125No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA157730.394325Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA148230.370575Illumina PCR Primer Index 6 (100% over 28bp)
GCTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA142380.35595Illumina PCR Primer Index 6 (96% over 29bp)
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT101880.2547No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCCCCATCAATATCTC91320.22829999999999998No Hit
CTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA88480.2212Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTCGGCACCATCAATATCTC83550.20887499999999998No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTAT80080.2002No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATATCT79210.19802499999999998No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTCGGCACCATCAATATCTCG74420.18605No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCCCCATCAATATCT68530.171325No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA61050.152625No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATG60590.151475No Hit
CTGTAAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA59260.14815Illumina PCR Primer Index 6 (100% over 28bp)
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTAT58500.14625No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTC58480.1462No Hit
CCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT58240.1456No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA57420.14354999999999998Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA56370.140925Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA56250.140625Illumina PCR Primer Index 6 (96% over 29bp)
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCT56160.1404No Hit
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG55170.137925Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCCTCAATATCTCG55020.13755No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCT54850.137125No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA49180.12294999999999999Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT46840.1171No Hit
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA43370.108425Illumina PCR Primer Index 6 (96% over 29bp)
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA41720.1043No Hit
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41370.103425Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTCTGCCGTCTTCTGCTTGAAAAAAAAAA40080.10020000000000001Illumina PCR Primer Index 6 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)