FastQCFastQC Report
Thu 22 Jun 2023
ERR2382495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102159
Total Bases13.8 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2631125.75495061619632Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA68146.669994812008731Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA66386.497714347243024Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA36693.591460370598772Illumina PCR Primer Index 6 (100% over 28bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG25242.4706584833445904No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC24252.373750721913879No Hit
GCTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA20391.9959083389618146Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA19421.9009583100852594Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCT15521.519200462024883No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14901.4585107528460537Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCG13181.2901457531886569No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA10200.9984436026194461Illumina PCR Primer Index 6 (96% over 29bp)
CTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA9760.955373486428019Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTC9290.9093667714053584No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8100.7928816844330895Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGT7880.771346626337376No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA7480.7321919752542605No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT7320.7165301148210143No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA6880.6734599986295873No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA6870.6724811323525093Illumina PCR Primer Index 6 (100% over 28bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA6190.6059182255112129Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATATCTCGT6170.6039604929570571No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA4110.40231403987901215Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA3890.3807789817832986Illumina PCR Primer Index 6 (96% over 29bp)
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT3790.37099031901251966No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3600.3523918597480398Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3100.3034485458941454Illumina PCR Primer Index 6 (100% over 28bp)
GCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATATCT2390.23394904022161533No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTAT2360.23101244139038168No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTAT2290.22416037745083645No Hit
GTATGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2200.21535058095713544Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA2160.2114351158488239No Hit
CGTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2040.19968872052388925Illumina PCR Primer Index 6 (96% over 29bp)
CCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT2010.19675212169265555No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTC2000.19577325541557766No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCCCCATCAATATCT1890.1850057263677209No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCT1800.1761959298740199No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT1700.16640726710324102No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1590.15563973805538425Illumina PCR Primer Index 6 (96% over 29bp)
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATG1560.15270313922415057No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA1530.14976654039291692No Hit
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG1520.148787674115839Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA1500.14682994156168325Illumina PCR Primer Index 6 (96% over 29bp)
AGCGGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1500.14682994156168325Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1350.13214694740551494Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATATCTCGTA1320.12921034857428126No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCT1280.1252948834659697No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1270.12431601718889183Illumina PCR Primer Index 6 (96% over 29bp)
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCG1240.12137941835765817No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATGC1150.11256962186395715No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA1140.11159075558687928No Hit
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1130.11061188930980138Illumina PCR Primer Index 6 (96% over 29bp)
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGT1130.11061188930980138No Hit
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG1130.11061188930980138No Hit
GTCTTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA1120.10963302303272349Illumina PCR Primer Index 6 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTC1100.10767529047856772Illumina Single End PCR Primer 1 (95% over 24bp)
CAATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1090.10669642420148982Illumina PCR Primer Index 6 (96% over 29bp)
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA1040.10180209281610039No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)