FastQCFastQC Report
Thu 22 Jun 2023
ERR2382496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438846
Total Bases58.8 Mbp
Sequences flagged as poor quality0
Sequence length45-135
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14542933.13895990848726Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA397079.048048746029359Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA290796.626242463187541Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA235825.373639044220524Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA207724.733323307037093Illumina PCR Primer Index 6 (100% over 28bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA63971.4576867511609997Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA38330.8734271247772566Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA30140.6868012924807335Illumina PCR Primer Index 6 (100% over 28bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA26650.6072745336632897No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG23680.5395970340392757No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA22680.5168099971288334Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCCTG22160.5049607379354033No Hit
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT20570.4687293492477999Illumina PCR Primer Index 6 (95% over 21bp)
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG18940.43158647908377884No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC18800.4283962939163169Illumina PCR Primer Index 6 (96% over 29bp)
GCGGCGCTACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT17580.4005961088855771Illumina PCR Primer Index 6 (96% over 27bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCC17550.39991249777826393No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA16450.37484675717677723Illumina PCR Primer Index 6 (96% over 29bp)
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT15550.35433842395737913RNA PCR Primer, Index 6 (95% over 22bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCC15110.34431212771678443No Hit
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14270.3251710167120129RNA PCR Primer, Index 37 (96% over 27bp)
TCCCGCTAGAGACCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT11390.2595443504099388Illumina PCR Primer Index 6 (95% over 21bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA11380.25931648004083435TruSeq Adapter, Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11310.25772138745710343Illumina PCR Primer Index 6 (96% over 29bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG11150.2540754615514326No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA10950.24951805416934414Illumina PCR Primer Index 6 (100% over 28bp)
CCCTCACTGGGGTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT10910.24860657269292646Illumina PCR Primer Index 6 (95% over 21bp)
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG10810.24632786900188222No Hit
ATCCGGGTAGGACCTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT9160.20872925809965226Illumina PCR Primer Index 6 (95% over 22bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA9100.20736203588502572Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8960.2041718507175638Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA8570.19528490632249126Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCCAATATCTCGTATGCCGTCTTCTGCTTGAAAA8370.1907274989404028Illumina PCR Primer Index 6 (96% over 30bp)
ATCCTGTCTGTGACGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT8320.18958814709488067Illumina PCR Primer Index 6 (95% over 21bp)
ACCTCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT8130.18525861008189662Illumina PCR Primer Index 6 (95% over 21bp)
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG7940.18092907306891257No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCT7910.1802454619615993No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCTGT7900.18001759159249486No Hit
GTCCTGGCGGGGAAGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT7850.17887823974697273Illumina PCR Primer Index 6 (95% over 21bp)
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA7650.17432083236488427Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGATTGGAAGCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT7580.1727257397811533Illumina PCR Primer Index 6 (95% over 21bp)
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA7510.17113064719742233Illumina PCR Primer Index 6 (96% over 29bp)
ATCCGGGTAGGACCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT7010.15973712874220114Illumina PCR Primer Index 6 (95% over 21bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAACAAA6950.1583699065275746Illumina PCR Primer Index 6 (96% over 29bp)
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCCT6930.15791416578936573No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA6850.15609120283653036Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA6850.15609120283653036Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCT6840.15586333246742592No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCCTGTAGGCACCA6680.15221740656175517No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCT6320.1440140732739959No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA6300.14355833253578704Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCC6040.137633702939072No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG5980.13626648072444547No Hit
GCGGCGCTACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT5940.13535499924802777Illumina PCR Primer Index 6 (96% over 28bp)
ATCCCTTAGCTCTCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT5540.12624018448385083Illumina PCR Primer Index 6 (95% over 21bp)
TCCACAGAATTCGCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT5470.12464509190011985Illumina PCR Primer Index 6 (95% over 21bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCTGTAGGCACCAT5420.12350574005459773No Hit
ACCTCCCCTGGAGCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT5290.12054342525624022Illumina PCR Primer Index 6 (95% over 21bp)
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA5280.12031555488713581RNA PCR Primer, Index 37 (96% over 27bp)
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAG5160.11758111045788273No Hit
TTCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCG5130.11689749935056945No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG5070.1155302771359429No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4890.11142861049206326Illumina PCR Primer Index 6 (96% over 29bp)
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCC4630.10550398089534825No Hit
CTGTAGGCACCATCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4570.1041367586807217RNA PCR Primer, Index 37 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)