FastQCFastQC Report
Thu 22 Jun 2023
ERR2382497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935534
Total Bases393.7 Mbp
Sequences flagged as poor quality0
Sequence length120-135
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA71329124.298509232051135Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC44971415.319665859772021No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1647305.611585490067565Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1431624.876863970916364Illumina PCR Primer Index 6 (100% over 28bp)
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC1181404.0244807248016885No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC789372.689016717231005No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA591492.0149315252352724Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA536741.828423721203706Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA499861.7027907017939496Illumina PCR Primer Index 6 (96% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT469081.597937547308258No Hit
TGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCC464291.5816202435400168No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT388161.3222807162172197No Hit
GTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC251170.8556194545864568No Hit
CTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC250440.853132683866036No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA205020.6984078535625886Illumina PCR Primer Index 6 (100% over 28bp)
ATAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA166210.5662002211522674Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA159460.5432061083264578Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA155540.5298524902113211Illumina PCR Primer Index 6 (96% over 29bp)
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC136920.46642280416442117No Hit
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATAGCTCGTATGC134700.4588602959461549No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG103590.35288298483342384No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC98950.3370766613502007Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA78640.2678899307587648RNA PCR Primer, Index 37 (96% over 27bp)
TATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATG76150.2594076580274662No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA67200.22891916768805948Illumina PCR Primer Index 6 (96% over 29bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA67010.22827192599370336No Hit
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA65020.22149292087913136TruSeq Adapter, Index 6 (96% over 29bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC58200.19826035058698008TruSeq Adapter, Index 6 (96% over 26bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA53860.18347598767379292Illumina PCR Primer Index 6 (100% over 28bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA49790.1696113892736381Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATATCGTATGC48510.16525102417481793No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA46550.15857421511724953Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA46090.15700720890986103No Hit
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG45810.15605337904449412No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC45070.1535325429717387No Hit
TTGGATTTGAGTAAGAGCATAGCTGTAGGGACCATCAATATCTCGTATGC42650.1452887277067818No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA41490.141337146835976Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA37620.12815385548251185Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC37580.12801759407317376No Hit
TATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCCGT37260.12692750279846868No Hit
TTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTT37040.126178065047109Illumina PCR Primer Index 6 (95% over 22bp)
TTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCCGTCT36650.1248495163060622Illumina PCR Primer Index 6 (95% over 21bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAACAAA36580.12461105883972048Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCCAATATCTCGTATGCCGTCTTCTGCTTGAAAA35890.12226054952863771Illumina PCR Primer Index 6 (96% over 30bp)
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATAGCTCGTATGC35770.12185176530062332No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA33760.11500462948138226Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA32880.1120068784759434Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA32050.10917945423217718Illumina PCR Primer Index 6 (96% over 29bp)
TGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG31660.10785090549113041No Hit
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA31620.10771464408179227Illumina PCR Primer Index 6 (96% over 29bp)
TGATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTAT31350.1067948795687599No Hit
TTTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCCGTC31070.10584104970339298No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC30180.10280923334561957No Hit
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA29640.10096970431955479RNA PCR Primer, Index 37 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)