FastQCFastQC Report
Thu 22 Jun 2023
ERR2382498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386715
Total Bases51.8 Mbp
Sequences flagged as poor quality0
Sequence length44-135
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4807012.430342758879279Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA372019.619745807636114Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA342198.848635299897857Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA218645.6537760366161125Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA200955.1963332169685685Illumina PCR Primer Index 6 (100% over 28bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA39061.0100461580233504No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA35820.926263527403902Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA29860.7721448612026945Illumina PCR Primer Index 6 (100% over 28bp)
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG23680.6123372509470799No Hit
TCCCGCTAGAGACCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT21000.5430355688297583Illumina PCR Primer Index 6 (95% over 21bp)
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC19700.5094190812355353No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG18190.47037223795301447No Hit
CCCTCACTGGGGTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT18140.46907929612246746Illumina PCR Primer Index 6 (95% over 21bp)
GCGGCGCTACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT17220.44528916644040184Illumina PCR Primer Index 6 (96% over 27bp)
GTCCTGGCGGGGAAGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT16930.43779010382322897Illumina PCR Primer Index 6 (95% over 21bp)
ATCCGGGTAGGACCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT16910.4372729270910101Illumina PCR Primer Index 6 (95% over 21bp)
ATCCTGTCTGTGACGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT15870.41037973701563163Illumina PCR Primer Index 6 (95% over 21bp)
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG15600.4033978511306776No Hit
ACCTCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT15100.3904684328252072Illumina PCR Primer Index 6 (95% over 21bp)
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC14050.3633166543837193No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCC13940.3604721823565158No Hit
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA13740.35530041503432763Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT13670.35349029647156177Illumina PCR Primer Index 6 (95% over 21bp)
CTGTAGGCACCATCACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA12620.32633851803007385RNA PCR Primer, Index 22 (96% over 27bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA12520.3237526343689797Illumina PCR Primer Index 6 (96% over 29bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC11850.3064272138396494TruSeq Adapter, Index 6 (96% over 26bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCCTG11800.3051342720091023No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCC11640.3009968581513518No Hit
ATCCCTTAGCTCTCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT11260.2911705002391942Illumina PCR Primer Index 6 (95% over 21bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA11090.2867744980153343Illumina PCR Primer Index 6 (96% over 29bp)
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT10850.2805683772287085RNA PCR Primer, Index 6 (95% over 22bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC10670.2759137866387391No Hit
ACCTCCCCTGGAGCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT10520.272034961147098Illumina PCR Primer Index 6 (95% over 21bp)
CTCGCCCCCGGGAGACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT10410.2691904891198945Illumina PCR Primer Index 6 (95% over 21bp)
ATCCGGGTAGGACCTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT9800.25341659878722056Illumina PCR Primer Index 6 (95% over 22bp)
CAAGCTGTAGGCACCCTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA9510.2459175361700477Illumina PCR Primer Index 6 (96% over 29bp)
TGGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCT9410.2433316525089536No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9280.2399700037495313Illumina PCR Primer Index 6 (96% over 29bp)
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG9150.23660835499010902No Hit
ATCCGATTGGAAGCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT9130.23609117825789017Illumina PCR Primer Index 6 (95% over 21bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA8810.22781635054238908Illumina PCR Primer Index 6 (96% over 29bp)
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG8600.2223859948540915No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8080.20893939981640225No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCCTGTAGGCACCA8050.20816363471807403No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCC7520.19445845131427536No Hit
ACCTCGCATCCTTCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT7400.19135539092096246Illumina PCR Primer Index 6 (95% over 21bp)
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG7380.19083821418874364No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA7020.18152903300880494Illumina PCR Primer Index 6 (96% over 29bp)
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCC6920.17894314934771086No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC6510.16834102633722509No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT6510.16834102633722509No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCTGTAGGCACCAT6230.16110055208616164No Hit
AAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA6150.15903184515728638Illumina PCR Primer Index 6 (96% over 29bp)
GCAGACTTAAGATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCA6080.15722172659452052No Hit
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA6000.15515301966564524Illumina PCR Primer Index 6 (96% over 29bp)
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG5970.15437725456731702No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCCT5930.15334290110287938No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCT5780.14946407561123826No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG5710.1476539570484724No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA5410.13989630606519013No Hit
GCGGCGCTACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT5290.13679324567187723Illumina PCR Primer Index 6 (96% over 28bp)
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC5280.1365346573057678No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA5170.13369018527856433Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA4980.12877700632248557Illumina PCR Primer Index 6 (96% over 28bp)
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4830.12489818083084442No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC4820.12463959246473502No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4720.12205370880364091No Hit
CTCCCTTGGCTTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCC4710.12179512043753152No Hit
ATCCGGGTGGGACCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT4700.12153653207142212Illumina PCR Primer Index 6 (95% over 21bp)
ATCCTGCTGGTGTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT4660.12050217860698448Illumina PCR Primer Index 6 (95% over 21bp)
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC4620.11946782514254684No Hit
AGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA4480.11584758801701511Illumina PCR Primer Index 6 (96% over 29bp)
GCCCCCTATGAGGAGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT4440.11481323455257747Illumina PCR Primer Index 6 (95% over 21bp)
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG4310.11145158579315517No Hit
ATCCTGTCTGTGACGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT4300.11119299742704575RNA PCR Primer, Index 6 (95% over 22bp)
CTGTAGGCACCATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4190.10834852539984227RNA PCR Primer, Index 40 (96% over 29bp)
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4120.10653840683707641No Hit
GCTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAA4110.10627981847096699Illumina PCR Primer Index 6 (96% over 28bp)
TGCGACTGTTAATCGCAAGATCGTGAGTTCACCCCTCACTGGGGTCGCCC4050.10472828827431055No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCAGCCCTCACTGGGGTCGCCC3990.10317675807765408No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCC3970.10265958134543526No Hit
TTGCATTTGTAATGCAAAGGTCGTTAGTTCATTTCTGACAGGTGGCACCC3950.10214240461321644No Hit
GGGGCGTGTGGTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCT3950.10214240461321644No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTTTTAACCGAAATGTCAG3940.10188381624710705No Hit
CTCGCCCCCGGGAGACCTGTAGGCACCATCAATATCTCGTATGCCGTCTT3920.10136663951488822Illumina PCR Primer Index 6 (95% over 22bp)
CCACATTGGTAATGTGGAGATCATCGGTTCATCCGATTGGAAGCACCCTG3920.10136663951488822No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA3900.1008494627826694Illumina PCR Primer Index 6 (96% over 29bp)
TTCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCG3900.1008494627826694No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA3880.10033228605045058Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3880.10033228605045058RNA PCR Primer, Index 37 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)