FastQCFastQC Report
Thu 22 Jun 2023
ERR2382499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3749812
Total Bases503 Mbp
Sequences flagged as poor quality0
Sequence length45-135
%GC21

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA91811324.484240809939273Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA76500920.401262783307537Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA57235315.263511877395452Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA38087110.157069207736281Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2568126.848663346322429Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA361480.96399499494908Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA348330.9289265701853853Illumina PCR Primer Index 6 (100% over 28bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA207120.5523476910309103Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCCTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA196580.5242396152127093Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA167820.4475424367941646RNA PCR Primer, Index 22 (96% over 27bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA146490.39065958506719806Illumina PCR Primer Index 6 (96% over 29bp)
AGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA100020.26673337223306126Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA99180.26449325992876443Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA97420.25979969129119007Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC91840.24491894526978952No Hit
GCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA90900.24241215292926688Illumina PCR Primer Index 6 (96% over 29bp)
AAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA82780.2207577339877306Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATAGCTCGTATGCCGTCTTCTGCTTGAAAAA75020.20006336317660725TruSeq Adapter, Index 6 (96% over 28bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA71560.19083623392319402Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA65460.17456875171341923Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA57870.1543277369638798Illumina PCR Primer Index 6 (96% over 28bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA57770.15406105692765398Illumina PCR Primer Index 6 (96% over 29bp)
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC57140.15238097269943135No Hit
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA55810.14883412821762798RNA PCR Primer, Index 37 (96% over 27bp)
CAAGCTGTAGGCACCGTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA54940.14651401190246338TruSeq Adapter, Index 6 (96% over 31bp)
TCAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA52220.13926031491712118Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA51330.13688686259471142TruSeq Adapter, Index 6 (96% over 29bp)
CTGTAGGCACCATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA50430.13448674226867907RNA PCR Primer, Index 40 (96% over 29bp)
GCTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAA45020.1200593523088624Illumina PCR Primer Index 6 (96% over 28bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA44080.11755255996833976Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA43890.1170458678995107Illumina PCR Primer Index 6 (96% over 29bp)
ATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA43440.11584580773649451Illumina PCR Primer Index 6 (100% over 28bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA41270.11005885095039432Illumina PCR Primer Index 6 (100% over 28bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA39310.10483192224036832Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA37760.10069838167886817RNA PCR Primer, Index 37 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)