FastQCFastQC Report
Thu 22 Jun 2023
ERR2382500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA52903313.225824999999999Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1238873.097175Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA958442.3961Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA553121.3828No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG470481.1762No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC455511.138775No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA430791.076975Illumina PCR Primer Index 6 (100% over 28bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC427661.06915No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG417811.044525No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT406631.016575No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA385290.963225No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC348340.8708499999999999No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG346400.8659999999999999No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC288300.72075No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG286140.71535No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA284110.710275Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCTGT262650.656625No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC256470.6411749999999999No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC255160.6379No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA232540.58135Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA229190.572975Illumina PCR Primer Index 6 (96% over 29bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC225180.56295TruSeq Adapter, Index 6 (96% over 26bp)
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG224230.5605749999999999No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCT215480.5387000000000001No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC186200.4655No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG177450.443625No Hit
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAG164330.410825No Hit
GCCCCCTATGAGGAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT160360.40090000000000003TruSeq Adapter, Index 6 (95% over 22bp)
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC153530.383825No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC150580.37645No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC145430.363575No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCTGTAGGCACCAT139620.34905No Hit
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT138190.345475RNA PCR Primer, Index 6 (95% over 22bp)
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC131590.328975No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA131010.327525No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA125880.3147Illumina PCR Primer Index 6 (96% over 29bp)
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCT116000.29No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC113230.28307499999999997No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC112730.281825No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC112330.280825No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA111950.279875No Hit
CTCGCCCCCGGGAGACCTGTAGGCACCATCAATATCTCGTATGCCGTCTT103880.2597Illumina PCR Primer Index 6 (95% over 22bp)
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC94630.23657499999999998No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC94140.23535No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCACTGTAGGCACCA93790.23447500000000002No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC86200.21549999999999997No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA85670.214175No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCT83490.20872500000000002No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCCA81520.20379999999999998No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCCTG80650.20162499999999997No Hit
GGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCTT80260.20065No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC80010.20002499999999998No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG77770.19442500000000001No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA77120.1928Illumina PCR Primer Index 6 (96% over 29bp)
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACCA74570.186425No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAAGTCCTGGCGGGGAAGCCT72890.182225No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA68830.172075Illumina PCR Primer Index 6 (96% over 29bp)
TGGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCT68750.171875No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCCTGTAGGCACC68350.170875No Hit
TCCCGCTAGAGACCACCTGTAGGCACCATCAATATCTCGTATGCCGTCTT67840.1696Illumina PCR Primer Index 6 (95% over 22bp)
GTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG67640.1691No Hit
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCA66260.16565No Hit
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCCTCTT64680.16169999999999998Illumina PCR Primer Index 6 (95% over 21bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA63780.15945Illumina PCR Primer Index 6 (96% over 29bp)
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCTGTAGGCACCATC63720.1593No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC62830.15707500000000002No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCC62720.1568No Hit
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG60150.150375No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCTG59510.148775No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT56650.141625No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGTGCCCCCTATGAGGAGCCA54380.13595000000000002No Hit
GCCCCCTACAGGGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC53890.13472499999999998No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA53670.13417500000000002Illumina PCR Primer Index 6 (100% over 28bp)
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC52600.1315No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGCGCCCCCTACAGGGCTCCA52140.13035No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCCATATCTCGTATGCCGTC50820.12705No Hit
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC50190.125475No Hit
TCCCGCTAGAGACCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT49460.12365Illumina PCR Primer Index 6 (95% over 21bp)
CCTGATTCAAGCTCAGGTATCGTAAGATGCACGAGTTCGAATCTCTTAGC48170.120425No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC47400.11850000000000001No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGGGCCCCCTACAGGGCTCCA46460.11615No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCCT46330.115825No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCAACCCTCACTGGGGTCGCCA46200.1155No Hit
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG46200.1155No Hit
TTCCGATATAGTGTAACGGCTATCACATCCCGCTTTCACCGTGGAGACCG45330.113325No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGAGCCCCCTATGAGGAGCCA45280.1132No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCATGTCCTGGCGGGGAAGCCT45070.112675No Hit
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCA44220.11055No Hit
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC44220.11055No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCACCCCTCACTGGGGTCGCCA43890.10972499999999999No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCCATATCTCGTATGCCGTC43140.10785No Hit
CAAGACTGTAAATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGACCA42870.107175No Hit
ATCCGGGTAGGACCTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT42540.10635Illumina PCR Primer Index 6 (95% over 22bp)
TTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC42410.106025No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCCTCAATATCTCGTATGCCGTC41750.104375No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCT40500.10124999999999999No Hit
TTGCATTTGTAATGCAAAGGTCGTTAGTTCATTTCTGACAGGTGGCACCA40280.10070000000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)