FastQCFastQC Report
Thu 22 Jun 2023
ERR2382504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068334
Total Bases145.2 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA20763219.43512047730391Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA15770114.761394844683403Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA12190511.410757309979838Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1046939.799650671044823Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA681556.379559201523119Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA241042.256223241046339Illumina PCR Primer Index 6 (100% over 28bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC161471.5114187136232675No Hit
CAATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA100220.9380961384735484Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA89520.8379401947331078Illumina PCR Primer Index 6 (96% over 29bp)
GCTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA88640.8297030703880995Illumina PCR Primer Index 6 (96% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT87020.8145392732984255No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA80670.7551009328543321Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA79760.7465829974521077Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA62140.581653303180466Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT58410.5467391283999199No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA54770.5126673867910223Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA54200.5073319767039147Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA48750.456317967976307Illumina PCR Primer Index 6 (96% over 28bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA40110.3754443834980446Illumina PCR Primer Index 6 (100% over 28bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA38410.35953175692246053Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA33870.3170356835970773Illumina PCR Primer Index 6 (100% over 28bp)
CTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA33290.311606669824231Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG31860.2982213427635927No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC30720.28755052258937747TruSeq Adapter, Index 6 (96% over 26bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAA30270.28333835673113467Illumina PCR Primer Index 6 (96% over 28bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA24750.23166912220335587Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA24340.22783137108806795Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG20840.19507008107951257Illumina PCR Primer Index 6 (96% over 29bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA19870.1859905235628558Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA19110.17887664344671236Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA18960.1774725881606314Illumina PCR Primer Index 6 (100% over 28bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA16550.15491409989759755Illumina PCR Primer Index 6 (100% over 28bp)
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG16430.15379085566873282No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15580.1458345423809408Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGAAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA14470.13544453326394182Illumina PCR Primer Index 6 (96% over 29bp)
CCTTCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC14370.13450849640655452No Hit
CAAGCAGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA14040.13141957477717642Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA14030.1313259710914377TruSeq Adapter, Index 6 (96% over 29bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC13700.1282370494620596No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT13180.12336965780364569No Hit
AGATCGGAAGAGCGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT13160.12318245043216822Illumina Single End PCR Primer 1 (95% over 23bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCCATATCTCGTATGCCGTC13140.12299524306069076No Hit
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT13130.12290163937495202No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA12770.11953190668835777Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCCTCAATATCTCGTATGCCGTC12520.11719181454488953No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA11980.11213721551499813Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA11960.11195000814352066Illumina PCR Primer Index 6 (96% over 29bp)
AGCGGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA11890.11129478234334954Illumina PCR Primer Index 6 (96% over 29bp)
GCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA11540.10801865334249403Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCGGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA11310.10586576857050324Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10720.10034315111191819Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)