FastQCFastQC Report
Thu 22 Jun 2023
ERR2382507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA122683330.670825Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA44729511.182375Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA43810510.952625000000001Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1182602.9565Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA909082.2727Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA759701.8992499999999999Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA651261.62815Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA507251.268125Illumina PCR Primer Index 6 (96% over 29bp)
GCTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA428221.07055Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA420911.052275Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA301560.7539Illumina PCR Primer Index 6 (96% over 29bp)
CTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA237720.5943Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA219790.5494749999999999Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCG205280.5132No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC195270.48817499999999997No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG191850.479625No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA191630.479075Illumina PCR Primer Index 6 (100% over 28bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA175790.439475Illumina PCR Primer Index 6 (96% over 29bp)
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA156010.390025No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA144940.36235No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA130340.32585000000000003Illumina PCR Primer Index 6 (100% over 28bp)
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA128370.32092499999999996No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT119430.29857500000000003No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGT117540.29385No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT114000.28500000000000003No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTC106890.267225No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT93640.23410000000000003No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCT85820.21455No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA82490.206225Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA70670.176675Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATATCTCGT69410.173525No Hit
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG64340.16085Illumina PCR Primer Index 6 (96% over 29bp)
CAATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA61940.15485Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCG61290.153225No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA59180.14795Illumina PCR Primer Index 6 (96% over 28bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA58230.145575Illumina PCR Primer Index 6 (96% over 29bp)
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA56170.140425Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA54840.1371Illumina PCR Primer Index 6 (96% over 29bp)
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA54120.1353No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATATCT51600.129No Hit
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA50090.125225Illumina PCR Primer Index 6 (96% over 29bp)
CCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT49550.123875No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT48990.12247499999999999No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAA48390.12097500000000001Illumina PCR Primer Index 6 (96% over 28bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA48120.1203Illumina PCR Primer Index 6 (96% over 29bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGT47540.11885000000000001No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA46980.11745No Hit
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA46960.11739999999999999Illumina PCR Primer Index 6 (100% over 28bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT46530.116325No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTAT45890.11472500000000001No Hit
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA43360.1084Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA43300.10824999999999999Illumina PCR Primer Index 6 (96% over 29bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA42230.105575Illumina PCR Primer Index 6 (96% over 29bp)
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA40970.102425No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATATC40690.101725No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA40470.101175Illumina PCR Primer Index 6 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)