FastQCFastQC Report
Thu 22 Jun 2023
ERR2382510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA118029629.5074Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA44089511.022375Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA43787410.94685Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1153902.8847500000000004Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA897572.243925Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA741411.8535249999999999Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA622301.55575Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA497721.2443Illumina PCR Primer Index 6 (96% over 29bp)
GCTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA476051.190125Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA402111.005275Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA298100.74525Illumina PCR Primer Index 6 (96% over 29bp)
CTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA216520.5413Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA215760.5394Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCG208900.52225No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC199280.49820000000000003No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG195740.48935No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA187510.468775Illumina PCR Primer Index 6 (100% over 28bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA169300.42325Illumina PCR Primer Index 6 (96% over 29bp)
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA161770.40442500000000003No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA152010.380025No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA133740.33435000000000004No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGT125190.312975No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA124270.31067500000000003Illumina PCR Primer Index 6 (100% over 28bp)
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT123570.308925No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT119950.299875No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTC106810.267025No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT99700.24925No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCT86870.217175No Hit
CTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA82080.2052Illumina PCR Primer Index 6 (100% over 28bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA81120.20279999999999998Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATATCTCGT71920.1798No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA70640.1766Illumina PCR Primer Index 6 (96% over 29bp)
CAATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA65750.164375Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG65640.16410000000000002Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCG63470.158675No Hit
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA57290.143225Illumina PCR Primer Index 6 (96% over 29bp)
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA56820.14205No Hit
CCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT54380.13595000000000002No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA53350.13337500000000002Illumina PCR Primer Index 6 (96% over 29bp)
GCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATATCT52160.13040000000000002No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA51750.129375Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA51030.127575Illumina PCR Primer Index 6 (96% over 29bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGT50460.12615No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT49930.124825No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA48730.12182499999999999Illumina PCR Primer Index 6 (100% over 28bp)
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTAT48310.12077500000000001No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA47810.11952499999999999No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA47190.117975Illumina PCR Primer Index 6 (96% over 29bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT46540.11635000000000001No Hit
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA46080.1152Illumina PCR Primer Index 6 (100% over 28bp)
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA42940.10735No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA42790.106975Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA42390.10597499999999999Illumina PCR Primer Index 6 (96% over 28bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAA42210.105525Illumina PCR Primer Index 6 (96% over 28bp)
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATATC41670.10417499999999999No Hit
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA40860.10215Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)