FastQCFastQC Report
Thu 22 Jun 2023
ERR2382511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA110379227.594800000000003Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA42173110.543275000000001Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2653846.6346Illumina PCR Primer Index 6 (100% over 28bp)
GCTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1612944.03235Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1115272.788175Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA894212.235525Illumina PCR Primer Index 6 (96% over 29bp)
CTGGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA712751.781875Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA703791.759475Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA462101.15525Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA379790.949475Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA337960.8449Illumina PCR Primer Index 6 (96% over 29bp)
CAATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA248580.6214500000000001Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCG205430.513575No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG187810.46952499999999997No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC186260.46565No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA180680.4517Illumina PCR Primer Index 6 (96% over 29bp)
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA159230.398075No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA152030.380075No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA149280.37320000000000003Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA134780.33695No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA133870.334675Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGT129070.322675No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT120020.30005000000000004No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT117430.293575No Hit
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA114050.285125Illumina PCR Primer Index 6 (100% over 28bp)
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT106630.266575No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTC103470.258675No Hit
AGCGGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA102390.255975Illumina PCR Primer Index 6 (96% over 29bp)
GTATGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA101470.253675Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCGGCTTGAAAAAAAA94180.23545Illumina PCR Primer Index 6 (96% over 28bp)
GTCTTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA84180.21045Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCT83100.20775No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA77280.19319999999999998Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATATCTCGT74920.18730000000000002No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA74580.18645Illumina PCR Primer Index 6 (100% over 28bp)
CGTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA69220.17305Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA66410.166025Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG63650.159125Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCG62620.15655No Hit
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA55790.139475No Hit
CCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT55490.13872500000000001No Hit
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA55200.13799999999999998Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTGTGCTTGAAAAAAA53240.1331RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA51770.129425Illumina PCR Primer Index 6 (96% over 29bp)
GCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATATCT50970.127425No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT48010.12002499999999999No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA47650.11912500000000001No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTAT47170.117925No Hit
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA47070.117675Illumina PCR Primer Index 6 (96% over 29bp)
TAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA46750.116875Illumina PCR Primer Index 6 (96% over 29bp)
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA44430.111075No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA44060.11015Illumina PCR Primer Index 6 (96% over 29bp)
CTTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA44030.11007499999999999Illumina PCR Primer Index 6 (100% over 28bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT42580.10644999999999999No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA42060.10515Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA41850.10462500000000001Illumina PCR Primer Index 6 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)