FastQCFastQC Report
Thu 22 Jun 2023
ERR2382512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases544 Mbp
Sequences flagged as poor quality0
Sequence length136
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA124424531.106125000000002Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA49378412.3446Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA43854110.963525Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1178742.94685Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA904502.26125Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA755571.888925Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA708381.77095Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA503281.2582Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA423711.059275Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA337650.8441250000000001Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA238780.59695Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA214290.535725Illumina PCR Primer Index 6 (100% over 28bp)
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCG203560.5089No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTC195320.4883No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCG189860.47464999999999996No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA177870.44467500000000004Illumina PCR Primer Index 6 (96% over 29bp)
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA156220.39055No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA146650.366625No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA138760.3469Illumina PCR Primer Index 6 (100% over 28bp)
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA132830.332075No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT120340.30085No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT116400.291No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGT109690.274225No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTC107200.268No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT86130.21532500000000002No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCT85010.212525No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA82220.20555Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA70600.17650000000000002Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG64240.1606Illumina PCR Primer Index 6 (96% over 29bp)
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATATCTCGT62750.15687500000000001No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCG62640.1566No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAA60560.1514Illumina PCR Primer Index 6 (96% over 28bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA59050.14762499999999998Illumina PCR Primer Index 6 (96% over 29bp)
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA55840.1396Illumina PCR Primer Index 6 (96% over 29bp)
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGTA55100.13775No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA54210.13552499999999998Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA52740.13185Illumina PCR Primer Index 6 (96% over 29bp)
GCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATATCT51720.1293No Hit
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA51450.12862500000000002Illumina PCR Primer Index 6 (96% over 29bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGT48560.12140000000000001No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT48230.120575No Hit
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA48100.12025Illumina PCR Primer Index 6 (100% over 28bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAA47900.11975Illumina PCR Primer Index 6 (96% over 28bp)
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTAT47560.11889999999999999No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTA46600.1165No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAT46450.11612499999999999No Hit
CCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATATCTCGT45600.11399999999999999No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA44910.112275Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA44670.111675Illumina PCR Primer Index 6 (100% over 28bp)
AGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGA43290.10822500000000002Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA43270.10817500000000001Illumina PCR Primer Index 6 (96% over 29bp)
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA43100.10774999999999998No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA42040.10510000000000001Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)