FastQCFastQC Report
Thu 22 Jun 2023
ERR2382514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3490844
Total Bases468 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA118631033.98347219182524Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA3427209.817683058882036Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA2819448.076671429602698Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1511644.330299492042612Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1407314.031431940241386Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA670981.922113964416628Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA319910.9164259416920377Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA310170.8885243797774979Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA310000.8880373915305295Illumina PCR Primer Index 6 (100% over 28bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC309790.8874358178136863No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA294640.8440365710985652Illumina PCR Primer Index 6 (100% over 28bp)
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG268350.7687252710232826No Hit
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC233170.6679473502683019No Hit
TCAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA228540.6546840821302814Illumina PCR Primer Index 6 (96% over 29bp)
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG226480.6487829304317236No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA212560.608907186915256Illumina PCR Primer Index 6 (96% over 29bp)
TGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG208300.5967038343735784No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA205420.5884536805425851Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA201090.5760498034286264Illumina PCR Primer Index 6 (96% over 29bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA184470.5284395406956025Illumina PCR Primer Index 6 (96% over 29bp)
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA148280.42476833682628046No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT134850.3862962653157804No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA105740.30290668961431677Illumina PCR Primer Index 6 (96% over 29bp)
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG73700.2111237282445162No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC69100.19794639920890195No Hit
GCAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA68990.1976312891667459Illumina PCR Primer Index 6 (96% over 29bp)
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCTGT67660.19382132229340526No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA65300.18706077957078576Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA62500.17903979667954226Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA61900.17732101463141867TruSeq Adapter, Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA60170.1723651930593289Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAA54860.15715397193343503TruSeq Adapter, Index 6 (96% over 28bp)
AAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA53240.15251326040350127Illumina PCR Primer Index 6 (96% over 29bp)
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG51740.14821630528319224No Hit
TTGGATTTGAGTAAGAGCAAGCTGTAGGCACCATCAATATCTCGTATGCC51340.14707045058444318No Hit
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT48800.1397942732473866No Hit
GCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG45030.1289945927116766No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC42840.12272103823602544Illumina PCR Primer Index 6 (96% over 29bp)
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG41100.11773657029646699Illumina PCR Primer Index 6 (96% over 29bp)
CTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC39310.11260887051956489No Hit
GTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC38630.11066091753169148No Hit
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA36800.10541863228491447TruSeq Adapter, Index 6 (96% over 29bp)
ATGGATTTGAGTAAGAGCAAGCTGTAGGCACCATCAATATCTCGTATGCC35080.10049145708029349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)