FastQCFastQC Report
Thu 22 Jun 2023
ERR2382516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4035990
Total Bases541.3 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC21

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA86152521.346063791040116Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA84434120.920294648896554Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA65331216.187156063320277Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA3970409.837487208838477Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3083017.638794942504814Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA749661.8574377042559571Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA450841.1170493484869883Illumina PCR Primer Index 6 (100% over 28bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA226260.5606059479830228Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCCTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA187290.4640497127098927Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA179990.44596245283065616RNA PCR Primer, Index 22 (96% over 27bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA172520.4274539827898483Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA134560.33340023141781816Illumina PCR Primer Index 6 (96% over 29bp)
AAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA112520.27879157282352035Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA98360.243707244071467Illumina PCR Primer Index 6 (96% over 29bp)
AGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA91520.22675972933530558Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA78260.1939053367327471Illumina PCR Primer Index 6 (100% over 28bp)
GCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA71190.17638794942504812Illumina PCR Primer Index 6 (96% over 29bp)
TCAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA68700.1702184594114455Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA66520.16481705851600226Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCTGTAGGCACCATCAATAGCTCGTATGCCGTCTTCTGCTTGAAAAA65420.1620915809999529TruSeq Adapter, Index 6 (96% over 28bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA58670.14536705987874102Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC58490.14492107264884205No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA58020.14375655043743915Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA57290.14194782444951548RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACCATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA55840.1383551495419959RNA PCR Primer, Index 40 (96% over 29bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA53320.1321113283234101TruSeq Adapter, Index 6 (96% over 29bp)
CAAGCTGTAGGCACCGTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA50400.12487642437171548TruSeq Adapter, Index 6 (96% over 31bp)
GCTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAA48960.12130852653252362Illumina PCR Primer Index 6 (96% over 28bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA47700.11818661592323074Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA47520.11774062869333175Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA44780.11095171197153611RNA PCR Primer, Index 37 (96% over 27bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA42530.10537687159779881Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC42150.1044353430013454Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)