FastQCFastQC Report
Thu 22 Jun 2023
ERR2382517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506704
Total Bases201.8 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA47122031.274888763818243Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA17460011.588208433773323Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA16916011.227155433316696Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1236488.206522316261191Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA647994.300712017755312Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA400742.659712856672578Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA219181.4546984676485892Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA150630.9997318650511314Illumina PCR Primer Index 6 (100% over 28bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA82550.54788465418556Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA76660.5087927024817084Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA63610.4221798043942274Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA56670.37611899882126815Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA39070.2593077339676539Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA37240.24716201722435197Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGTCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA34500.22897662712782338TruSeq Adapter, Index 6 (96% over 29bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA29530.1959907188140471Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA26570.17634518790684833Illumina PCR Primer Index 6 (96% over 29bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA24160.160350009026325Illumina PCR Primer Index 6 (96% over 29bp)
AGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA23180.15384574541515786Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA21240.14096995826652084Illumina PCR Primer Index 6 (100% over 28bp)
AAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA20200.13406747443426179Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA20190.13400110439741314Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA20060.13313829391838078Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA18350.12178901761726259Illumina PCR Primer Index 6 (100% over 28bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA18300.12145716743301938Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA17570.1166121547430683Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA17340.1150856438955495Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA17180.11402372330597119RNA PCR Primer, Index 32 (96% over 28bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA16010.10625842899467978Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA15850.10519650840510146TruSeq Adapter, Index 6 (96% over 29bp)
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA15720.1043336979260691Illumina PCR Primer Index 6 (96% over 29bp)
GCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA15540.10313903726279348Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)