FastQCFastQC Report
Thu 22 Jun 2023
ERR2382519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441403
Total Bases70.9 Mbp
Sequences flagged as poor quality0
Sequence length35-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA16113511.179038755989824Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA15008810.412632691898102Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA1184808.219769210970144Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA604284.192304303515394Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA592264.108913329582358Illumina PCR Primer Index 6 (100% over 28bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACC435033.018101113984083No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCC425852.9544131655061077No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCC258921.7963054052197756No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT231901.608849155995929No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC163071.131328296111497No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCC141530.9818905607938931No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCC135750.9417907413818342No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG132480.9191045113684376No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC103400.7173566310046531No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA103120.7154140791992247Illumina PCR Primer Index 6 (100% over 28bp)
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG74390.5160943885922258No Hit
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGC71170.49375504282979843No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC56620.3928117257977124No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCC55080.38212769086785586No Hit
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCC54810.3802545159126212No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCC52020.36089837470853053No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC45900.3184397423898799No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG43040.29859796323443205No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCCTG41840.2902727412111672No Hit
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCC39750.27577297952064755No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCACCCCTCACTGGGGTCGCC37680.26141197153051576No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC37300.25877565122314855No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG36860.2557230698146181No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCC36260.25156045880298566No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA34900.2421252071766189Illumina PCR Primer Index 6 (96% over 29bp)
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG32420.22491974832853823No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA31980.2218671669200078Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGA31320.21728829480721212Illumina PCR Primer Index 6 (96% over 29bp)
TGCGACTGTTAATCGCAAGATCGTGAGTTCAACCCTCACTGGGGTCGCC31120.21590075780333465No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA30740.2132644374959675Illumina PCR Primer Index 6 (96% over 29bp)
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCC30480.21146063939092674No Hit
CTCCCTTGGCTTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCC30430.2111137551399574No Hit
TTCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCG30240.2097955949862738No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAA28460.1974465156517643Illumina PCR Primer Index 6 (96% over 28bp)
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG28150.1952958332957542No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCC27720.19231262873741764No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCC25990.1803104336538775No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA25670.17809037444767356Illumina PCR Primer Index 6 (96% over 29bp)
TGCGACTGTTAATCGCAAGATCGTGAGTTCAGCCCTCACTGGGGTCGCC24980.17330337178429628No Hit
TGGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCT24740.17163832737964332No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCACTGTAGGCACC24320.1687244996715006No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA23720.1645618886598682Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA23310.16171743780191938Illumina PCR Primer Index 6 (100% over 28bp)
AGATCGGAAGAGCGTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT22700.1574854499400931Illumina Single End PCR Primer 1 (95% over 23bp)
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC22660.15720794253931758No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT22530.15630604348679725No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGTGCCCCCTATGAGGAGCC21660.15027025751993023No Hit
CAAGACTGTAAATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGACC21590.1497846195685731No Hit
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG20670.14340194935073675No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA20530.14243067344802252Illumina PCR Primer Index 6 (96% over 29bp)
GCAGACTTAAGATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGC19470.13507672732747192No Hit
GTCTAGTCGGTTATGGCATCTGCTTGACACGCAGAACGTCCCCAGTTCGT19310.13396669772436995No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC19230.13341168292281896No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGTGCTCC18910.131191623716615No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCTGTAGGCACCAT18500.12834717285866618No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC17730.12300515539373791No Hit
GTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTT17710.12286640169335017No Hit
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC17690.1227276479929624No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTTCTTGAAAAA17230.11953631288404423TruSeq Adapter, Index 6 (96% over 28bp)
CAAGACTGTAAATCTTGAGATCGGGCGTTCGGCTCGCCCCCGGGAGACC17150.11898129808249323No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA16960.11766313792880964Illumina PCR Primer Index 6 (96% over 29bp)
GTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCC16070.1114885982615549No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCCT15870.11010106125767742No Hit
TGGCCATCTTAGTATAGTGGTTAGTACACATCGTTGTGGCCGATGAAACC15610.1082972631526367No Hit
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC15350.10649346504759598No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGGGCCCCCTACAGGGCTCC15180.10531405859430013No Hit
GTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG14500.10059643278111674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)